مطالعه گونه های مختلف بیماری سل به روش انگشت نگاری ژنتیکی

یعقوبی, سجاد and مصوری, نادر and کوثرزاده, حنا (1391) مطالعه گونه های مختلف بیماری سل به روش انگشت نگاری ژنتیکی. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Genetic Fingerprinting in the Study of Tuberculosis Transmission

English Abstract

Background & Objectives: One of the potent tools to control Mycobacterium tuberculosis is molecular epidemiology techniques. RFLP-IS6110 standard Technique to genotyping M. tuberculosis.The aims of this study were to Identification the genetic diversity of M. tuberculosis population in markazi Province, and to recognition the manner of transmission of the disease in this region. Methods: RFLP was analyzed among 42 isolates of M. tuberculosis deposited in the Mycobacterial Centre from Markazi province . DNAs isolated from these strains were restricted with Pvu II , hybridized with a PCR amplified DIG-labeled 245 bp IS6110 probe. Results: Copy number higher than 17 however was not observed in any of the isolates studied., 72 percent of the isolates showed high copy number of IS6110, 13 per cent showed intermediate copy number, 10 percent showed low copy number, where as 5 percent of isolates lacked IS6110 element. Conclusion: IS6110 DNA fingerprinting helped us to understanding epidemiological links between some TB cases and that this technique estimate from reactivation of latent infection transmission of the disease in Markazi Province.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :سجاد یعقوبی
کلیدواژه ها (انگلیسی):DR ; IS6110 ; Mycobacterium tuberculosis ; RFLP
Subjects:QW Microbiology and Immunology
WC Communicable Diseases
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5034
Deposited By: MS Soghra Golmaghani
Deposited On:18 Sep 1393 12:56
Last Modified:18 Sep 1393 12:56

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