بررسی اثرات ضد باکتریایی روغن فرار گلپر (Heracleum persicum) بر روی برخی از باکتری های گرم مثبت و گرم منفی

عنوان انگليسی

The Antibacterial Effects of the Volatile Oil of Heracleum persicum on Some Gram-Positive and Gram-Negative Bacteria

خلاصه انگلیسی

Background & Objectives: Due to increased bacterial resistance to common antibiotics, tendency towards using herbal essential oils like Heracleum persicum is increasing. In this study, antibacterial effects of hydrodistilled volatile oil from aerial parts of Heracleum persicum were evaluated on several strains of Gram-positive and Gram-negative bacteria. Methods: This descriptive study was performed in department of microbiology. The hydrodistilled volatile oil from aerial parts of Heracleum persicum was analyzed by gas chromatography and mass spectrometry (GC-MS) technique. The antimicrobial activity of the aerial parts essential oil of Heracleum persicum was screened by disc diffusion and microdilution Methods was used for determination of MIC. The bacterial strains were Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, P. aeruginosa ATCC27853 and clinical isolates of S. typhimurium, E. aerugenes, S. epidermidis, Acinetobacter baumanii. Filter paper discs were impregnated with 100μl of volatile oil of Heracleum persicum to give a final concentration of 1mg/disc for disc diffusion. The inoculum size of strains was 1.5×108 cfu/ml. Inhibition zones were measured after 24hr incubation at 37°C. The serial dilutions of the oil from 2 to 512μg/ml were prepared in 96 wells microtitere plates using Muller Hinton broth for microdilution. 100μl of bacterial suspension in 1.5 × 105 cfu/ml concentration was added to each well. Incubation period was 18hr at 35°C. Results: Twenty compounds of the hydrodistilled volatile oil from aerial parts of Heracleum persicum were identified, representing 92.15 % of the total oil. (E)-anethole(23.0%), hexyl butanoate (19.7%) were the main components. the MIC varied from 26.67 to 512 μg/ml for all Gram-positive and Gram-negative bacteria. The highest MIC was observed against S. aureus (26.67μg/ml). The lowest MIC value 512 μg/ml was recorded against E.aerugenes. Conclusion: Result shows that the hydrodistilled volatile oil of Heracleum persicum could inhibit the growth of Gram-positive and Gram-negative bacteria. However, the inhibitory effect of Heracleum persicum on Gram-positivecocci is more than Gram-negative rods.

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