تعیین تیپ جدایه های استافیلوکوکوس اورئوس حفره بینی اسبان سالم با استفاده از ناحیه x ژن spa در ایران

عنوان انگليسی

Typing of Nasal Carriage Isolates of Staphylococcus aureus from Healthy Horses Using the PCR-RFLP

خلاصه انگلیسی

Background & Objectives: Staphylococcus aureus is an important pathogen that can colonize the nares of different animals. The aim of the present study was to investigate the genetic diversity of 24 S. aureus isolates recovered from the nose of healthy horses in Iran by molecular typing based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the protein A gene (spa). Methods: The polymorphic X region of the spa gene was amplified by PCR. The amplicons were then digested with restriction enzyme Hin6I and the restriction fragments were resolved by agarose gel electrophoresis. Results: All isolates gave a single PCR product ranging in size from approximately 1100 bp to 1400 bp. Among 24 S. aureus isolates studied, a total of 6 distinct RFLP patterns, designated as A-F, were observed. For the spa gene, the lack of amplification was also considered a distinct genotype (G). The genotyping results showed that 9, 1, 4, 4, 1, 4, and 1 isolates belonged to patterns A-G, respectively. Conclusion: The results of the study suggest that based on spa gene RFLP analysis, nares of healthy horses could be a reservoir of several genetic variants of S. aureus, with implications in public health.

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