title

بیان، تخلیص و بررسی آنتی ژنیسیته ناحیه آنتی ژنیک پروتئین cagA هلیکوباکتر پیلوری

فرجادی, وحیده and ابطحی, حمید and ذولفقاری, محمدرضا and صوفیان, صفیه and حسن زاده, لیلا (1391) بیان، تخلیص و بررسی آنتی ژنیسیته ناحیه آنتی ژنیک پروتئین cagA هلیکوباکتر پیلوری. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Title

Expression, Purification and Evaluation of Antigenicity for CagA Antigenic Fragment of Helicobacter pylori

English Abstract

Background & Objectives: Helicobacter pylori is one of the most common chronic infections worldwide. The cytotoxin-associated gen A (CagA) is important virulence determinant involved in gastric injury. The aim of the present study was to construct a recombinant protein containing antigenic region of cagA from H. pylori and determining its antigenicity as a vaccine candidate of H. pylori. Methods:The antigenic region of cagA gene was detected by bioinformatics. The target gene encoding cagA high antigenic region amplified from H. pylori chromosome by PCR, digested by BamHI and XhoI restriction endonuclease enzyme and inserted into the prokaryotic expression vector pET32a. The target protein was expressed in the E.coli BL21 (DE3) pLYsS. The expressed protein was purified by affinity chromatography with Ni-NTA resin. The integrity of the product was confirmed by western-blot analysis using Sera of infected individual. Results: Enzyme digestion analysis, PCR and sequencing showed that the target gene (1245 bp) was inserted correctly into the recombinant vector. The expressed protein was purified by affinitychromatography by Ni-NTA resin. The data also indicated that cagA protein from Helicobacter pylori recognized by patient sera. Conclusion: Results indicates that antigenic region of recombinant cagA protein (65kDa) were recognized as an antigen by patient sera, so it might be a candidate for the development of H. pylori vaccine and ELISA kits and serological diagnosis of H. pylori infections.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :وحیده فرجادی
کلیدواژه ها (انگلیسی):Antigenic Region ; Antigenicity ; CagA Gene ; Helicobacter pylori
Subjects:QW Microbiology and Immunology
WI Digestive System
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5052
Deposited By: MS Soghra Golmaghani
Deposited On:03 Oct 1392 08:48
Last Modified:31 Mar 1393 08:58

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