فعالیت آنتی باکتریایی عصاره های هیدروالکلی گل گاوزبان

Title

Antibacterial Activity of Hydro Alcoholic Extracts of Borago officinalis

English Abstract

Background & Objectives: Borago officinalis is an annual herb naturalized throughout the Mediterranean region, as well as Asia Minor, Europe, North Africa, and South America. It grows to a height of 60–100 cm (2.0–3.3 ft), and is bristly or hairy all over the stems and leaves; the leaves are alternate, simple, and 5–15 cm (2.0–5.9 in) long. The flowers are complete, perfect with five narrow, triangular-pointed petals. Flowers are most often blue in color, although pink flowers are sometimes observed. This study was designed to examine in vitro anti-bacterial potential of methanolic and ethanolic extract of B.officinalis. Methods: The inhibitory effect of methanolic and ethanolic extract of B.officinalis was tested against 3 Gram positive: Bacillus cereus, Staphylococcus aureus and Staphylococcus epidermidis and 5 Gram negative: Salmonella typhi, Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis ATCC bacterial species by disc diffusion methods at various concentrations from 600 mg ̸ml to 50 mg ̸ml. The viability of bacterial species was analyzed by susceptibility test (minimum inhibitory concentration and minimum bactericidal concentration). Results: The extract of B.officinalis was active against: Bacillus cereus, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli. The zone of inhibition for these bacterial species for methanolic extract was 8, 10, 19, 6mm. and about ethanolic extract was 8, 12, 20, 12mm. MIC and MBC for Staphylococcus aureus and Staphylococcus epidermidis for methanolic extract were 80mg/ml-160mg/ml and 80mg/ml-160mg/ml respectively. Conclusion: This extract can be used as antibacterial substance against some bacterial species such as Staphylococcus aureus and Staphylococcus epidermidis.

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