بررسی ژن های اینتگرون عامل مقاومت داروئی در نمونه های سودوموناس آئروژنوزای ایزوله شده از بیماران مبتلا به عفونت ادراری از بخش های پر خطر بیمارستان های تبریز

تقی زاده, بهارک and حسنی, اکبر and حسنی, آلکا and ورشوچی, مجتبی and دهقانی, لیلا and حاجی زاده, مریم (1391) بررسی ژن های اینتگرون عامل مقاومت داروئی در نمونه های سودوموناس آئروژنوزای ایزوله شده از بیماران مبتلا به عفونت ادراری از بخش های پر خطر بیمارستان های تبریز. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Detection of Integron Genes Responsible for Antibiotic Resistance InPseudomonas Aeruginosa Isolated from Urinary Tract Infections of Patients Admitted to High Risk Wards of Hospitals of Tabriz

English Abstract

Background & Objectives: Pseudomonas aeruginosa is one of the most important causative agents among hospital acquired infections, especially in ICU and burn units.The organism is known to be inherently resistance to a wide variety of antimicrobials. Integrons have been recognized as important contributor to the acquisition and dissemination of antibiotic resistance gene in Gram-negative bacteria. Investigate the resistance profiles of Pseudomonas aeruginosa isolated from urine of patient with Urinary Tract Infections (UTI) to 13 antibiotics, and its relation to presence of various class of integrons. Methods: In total , 65 clinical P. aeruginosa were isolated from urine of patients with UTI over a period of 10 months. All clinical samples were initially identified with standard phenotypic methods and then studied for their resistance pattern toward 13 antibiotics by disc diffusion methods according to CLSI recommndations. Presence of Class 1, 2 and 3 integrons was assessed by performing PCR. Results: The resistance rate were between 23,6% for meropenem and 100% for rifampicin. PCR results showed that 36 (55.38 %) of P. aeruginosa isolates carried class 1 integron. None of the isolates detected class 2 and3 integron genes. The amplicon size of integron 1 was 457 bp. Resistance towards antibiotics associated with the presence of Class 1 integron by PCR. Class 1 integron was found in 29 strains revealing resistance to ceftriaxone , rifampicinand 20strain revealing cefepime , ceftazidime , carbenicillin and 19 resistance to ampicillin/sulbactam respectively. Conclusion: Our study indicate that Class 1 integrons are widespread in P. aeruginosa isolated from clinical samples of University teaching hospitals of Tabriz. The antibiotic resistance rates in class 1 integron-positive strains of P. aeruginosa were noticeably higher than those in class 1 integron-negative strains.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :بهارک تقی زاده
کلیدواژه ها (انگلیسی):Pseudomonas aeruginosa ; Antibiotics ; Multidrug Resistance ; Integron
Subjects:QW Microbiology and Immunology
WJ Urogenital System
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5086
Deposited By: MS Soghra Golmaghani
Deposited On:03 Oct 1392 09:05
Last Modified:23 Mar 1397 12:00

Repository Staff Only: item control page

Document Downloads

More statistics for this item...