بررسی تولید پروژنی ویروس آنفلوانزا در فواصل زمانی مختلف در سلول های لاین کلیه سگ

عبدلی, اصغر and سلیمان جاهی, حوریه and توسطی خیری, معصومه and جمال, عباس and جماعتی, اعظم (1391) بررسی تولید پروژنی ویروس آنفلوانزا در فواصل زمانی مختلف در سلول های لاین کلیه سگ. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Determining Influenza Virus Shedding in Different Time Points in Madin Darby Canine Kidney Cell Line

English Abstract

Background & Objectives: Since cell supernatants are harvested after culture and tested for the presence of influenza infectious viruses, the residual infectious viruses may still be present immediately after virus inoculation and washing cells. This observable fact may lead to a false positive in reporting virus infectivity assay. Methods: In the present research, influenza virus progeny production in Madin Darby canine kidney (MDCK) cells with Multiplicities of Infection (MOI) in different time points was investigated and the results were analyzed by end point tests and immune florescence assay. Result: The amount of residual virus could be influenced by the MOI, virus ligands and cell receptors affinity, cell density and permissiveness on the virus attachment level. When the higher amount of virus MOI used, we can get much higher titers of residual virus. These effects are probably reflecting the presence of unattached viruses to cell receptors or perhaps rebounded viruses from cell receptors. Conclusion: In conclusion, the cell-based procedures proved to be useful tools for monitoring of influenza virus switching from transcription to replication and release of the virus particles. They cannot be carried out rapidly, but are as sensitive and specific as the molecular assays.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :اصغر عبدلی
کلیدواژه ها (انگلیسی):Influenza Virus ; MDCK ; Shedling
Subjects:QW Microbiology and Immunology
WC Communicable Diseases
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5091
Deposited By: MS Soghra Golmaghani
Deposited On:03 Oct 1392 09:53
Last Modified:03 Oct 1392 09:53

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