جداسازي و تشخيص بيش از يك گونه انگل لیشمانیا در افراد مشکوک به بیماری لیشمانیوز جلدی در کانون اندمیک ابرکوه یزد

میرزایی, هانیه and پرویزی, پرویز and امیر مظفری, نور and زمانی, علیرضا (1391) جداسازي و تشخيص بيش از يك گونه انگل لیشمانیا در افراد مشکوک به بیماری لیشمانیوز جلدی در کانون اندمیک ابرکوه یزد. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Detection and Identification of More Than One Speices of Leishmania in Human from Endemic Focus of Zoonotic Cutaneous Leishmaniasis in Abarkouh

English Abstract

Background & Objectives: Leishmaniasis is one of six important diseases which are great problems in global public health. In Iran, 15 out of 32 provinces have endemic regions for zoonotic cutaneous leshmaniasis (ZCL) where L. major have been reported as the main causative agent of this form of disease. Methods: Samples were collected from suspected people to ZCL in different villages and cities of Abarkouh in 2010 and 2011. Stained Smears were examined under a light microscope and classified based on number of Leishmania amastigotes. DNA of parasite extracted and ITS-rDNA gene amplified by PCR. The PCR product digested with BsuRI restriction enzyme which was selected based on in-silico analysis using CLC DNA software. Results: Samples were collected from lesions of 85 suspected people, 62 slides were microscopy-positive, 58 out of 85 were positive after amplifying ITS-rDNA gene. Using RFLP, most of the samples identified as L. major and those identified as non-L. major, were sent for sequencing and all Leishmania species will confirm in congress. Conclusion: According to our finding, Leishmania major has been definitely recognized as the causative agent of ZCL in Abarkouh. The exact identification of Leishmania species in human cases is necessary for treating and controlling disease to help local health authorities

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :هانیه میرزایی
کلیدواژه ها (انگلیسی):Leishmania major ; ITS ; rDNA ; PCR_RFLP ; Abarkouh ; Iran
Subjects:QW Microbiology and Immunology
QX Parasitology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5106
Deposited By: MS Soghra Golmaghani
Deposited On:03 Oct 1392 12:33
Last Modified:01 Apr 1393 05:03

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