title

سنتز نانو ذرات نقره با رامنولیپید بدست آمده از سودوموناس آئروژینوزا

ابراهیمی, زهرا and خداوردی, ابراهیم and شرفی, حکیمه and شهبانی ظهیری, حسین and فقیهی, شهاب and اکبری نوقابی, کامبیز (1391) سنتز نانو ذرات نقره با رامنولیپید بدست آمده از سودوموناس آئروژینوزا. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Title

Synthesis of Silver Nanoparticles by Glycolipid Biosurfactant Produced from Pseudomonas aeruginosa MA01

English Abstract

Background & Objectives: Nanotechnology involving the production, manipulation and use of material ranging in size from less than a micron to that of individual. Nanoparticles are material with at least one dimension roughly between 1 and 100 nm. Methods: The present study reports the synthesis of silver nanoparticles from biosurfactant. The surfactants are emerging as potential nanoparticle stabilizing agents.Therefore, the biosurfactants are described as a green alternate for the synthesis and stabilization of nanoparticles. It was found that the nanoparticles silver can be synthesized in reverse micelles using the glycolipid as stabilizer. The silver nanoparticles synthesized in this study were spherical with average diameter of 11nm for silver. Therefore, the biosurfactant-mediated nanoparticles synthesis can be considered as “green” stabilizer of nanoparticles. Results: A new bacterial strain isolated from spoiled fruits, identified as Pseudomonas aeruginosa MA01 that is capable of producing rhamnolipids. Rhamnolipid is composed of rhamnose sugar molecule and b-hydroxyalkanoic acid. An optimum biosurfactant production of 25 g/l was obtained with the following medium composition: 35ml acidified soybean oil, 3g/l NaNO3, 0.25g/l KH2PO4, 0.25g/l MgSO4 7H2O and 1g/l yeast extract, with shaking at 200 rpm for an incubation period of 14 days at 30 °C. The production of the biosurfactant was found to be a function of cell growth, with maximum production occurring during the exponential phase. The crude extract of biosurfactant was purified and fractioned using column chromatography. Two major fractions were obtained and each fraction was analyzed by analytical Thin Layer Chromatography (TLC) and revealed the presence of two glycolipid compounds. Conclusion: Although nanomaterials could be synthesized by using chemical approaches, it is now possible to include the use of biological materials. In this study we critically assess the role of microorganisms in the synthesis of nanoparticles.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :زهرا ابراهیمی
نویسنده مسئول :کامبیز اکبری نوقابی
کلیدواژه ها (انگلیسی):Silver Nanoparticles; Glycolipid Biosurfactant; Pseudomonas aeruginoa
Subjects:QW Microbiology and Immunology
WD Nutrition Disease and metabolic diseases
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5112
Deposited By: MS Soghra Golmaghani
Deposited On:04 Oct 1392 08:54
Last Modified:04 Oct 1392 08:54

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