جداسازی و شناسایی سویه های بومی انسیفر ملیلوتی از استان قم و خراسان رضوی

حداد سبزوار, آزاده and نخعی مقدم , محبوبه (1391) جداسازی و شناسایی سویه های بومی انسیفر ملیلوتی از استان قم و خراسان رضوی. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Isolation and Characterization of Native Strains of Ensifer Meliloti From Provinces of Qom Province and Khorasan Razavi

English Abstract

Background & Objectives: Nutrient enrichment in soil by N2-fixing bacteria has been known for many years.Rhizobia are the most well known species bacteria that infect plant roots of legumes to fix nitrogen. Due to the increasing use of chemical fertilizers and their detrimental effects on human and environment, the aim of this study was isolation and identification of indigenous bacteria of Ensifer meliloti from some are as of Qom and Khorasan Razavi. Methods: Bacteria were isolated from active nodules on the roots of perennial alfalfa (Medicago sativa) from farms in Qom and Khorasan Razavi. Suspensions of washed and disinfected nodes were inoculated on YEM (Yeast extract agar). Isolates were confirmed with nodulation test on alfalfa seeds varieties of Hamedani.Then bacteria were studied by some microscopic and biochemical tests such as gram reaction, indicator absorption in CRYMEN, growth in litmus milk and antibiotic sensitivity patterns. Results: 40 bacteria were isolated from plants,all of which were gram-negative bacilli or coccobacilli with milky to cream mucoid colonies. Bacteria were catalase+,oxidase+ and motile.They were grown in the presence of cogo-red and their identification was easier in CRYEM due to reduced growth of saprophyte and to absorbing reagent. Some isolates produced acid in litmus milk. All bacteria were susceptible to tetracycline and streptomycin, some were sensitive to nalidixic acid and vancomycin and 29 were resistant to penicillin. Conclusion: All bacteria were created nodules in root of alfalfa seed crops. Since Ensifer meliloti can strengthen soil and enhance the product naturally,isolation and identification of native strains could bea trigger for their mass production and inoculation to soil without Rhizobia to reduce the consumption of chemical fertilizers.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :آزاده حداد سبزوار
نویسنده :محبوبه نخعی مقدم
کلیدواژه ها (انگلیسی):Ensifer meliloti ; Nitrogen Fixation ; Alfalfa ; Antibiotic Resistance
Subjects:WA Public Health > WA 30 Social, Economic, and Environmental factor in public health
WA Public Health > WA 30 Social, Economic, and Environmental factor in public health

QW Microbiology and Immunology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5117
Deposited By: MS Soghra Golmaghani
Deposited On:05 Oct 1392 09:05
Last Modified:05 Oct 1392 09:05

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