تعیین فراوانی ایزوله های مولد MBLs) Metalo Beta-lactamase) در بین سودوموناس آئروجینوزاها ی جداسازی شده از نمونه هاي باليني در شهرستان ارومیه

Title

Determination the Frequency of MBL (Metalo Beta-Lactamase) Producing Pseudomonas Aeruginosa Isolated from Urmia Hospitals

English Abstract

Background & Objectives: Detection of metallo-β-lactamase producing P. aeruginosa is crucial for the optimal treatment of patients, however there are limited studies on metallo-β-lactamase producing P. aeruginosa isolates from West Azarbayejan, Iran. This study has been designed to detect the metallo-β-lactamase in P. aeruginosa isolates. Methods: 100 isolates were collected from clinical specimens submitted to hospital diagnostic laboratories in Urmia/Iran from July to September 2010. The susceptibilities of isolates to different classes of antibiotics were tested using agar disk diffusion Methods. All isolates of P.aeruginosa were subjected to determine MICs against Imipenem. Imipenem non-susceptible isolates were investigated for metallo-β-lactamase production by the combined disk Methods. Results: The rates of resistances were determined to antibiotics as follows: kanamycin(91 %),Tobramycin(34%), Ciprofloxacin(16%), Colistin(68%), Ticarcillin (46%), Amikacin(16%), Norfloxacin(23%), Gentamicin(33%), Ceftazidime (62%), Ceftizoxime (69%), and Cefepime (39%). 79 isolates (79%) were sensitive(MIC≤4mg/L) and 21 isolate(21%) were resistant to Imipenem(MIC≥8mg/L). the rate of resistance to different antibiotics were much higher in imipenem resistant isolates. Detection of metallo-β-lactamase producing isolates among imipenem non-susceptible isolates of P. aeruginosa revealed that 7 isolates (33.3%) were metallo-β-lactamase positive. Conclusion: Metallo-β-lactamase positive isolates showed very high resistances to all tested antibiotics. This result suggests that metallo-β-lactamase producing isolates in hospitals may cause serious infections lead to the patient fails antibiotic therapy.

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