title

تعیین فعالیت ضد میکروبی 5 عامل آنتی میکروبیال بر علیه ایزوله های کلینیکی کلستریدیوم دیفیسیل

اصلانی, محمد مهدی and گودرزی, مهدی and آل بویه, مسعود and عظیمی, معصومه and شایگان مهر, فرحناز سادات and گودرزی, حسین (1391) تعیین فعالیت ضد میکروبی 5 عامل آنتی میکروبیال بر علیه ایزوله های کلینیکی کلستریدیوم دیفیسیل. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Title

Minimum Inhibitory Concentration of 5 Antimicrobial Agents Against Different Clostridium difficile Clinical Isolates

English Abstract

Background & Objectives: Clostridium difficile is gram-positive rod, spore forming, strict anaerobic bacillus and the major cause of nosocomial diarrhea. C.difficile is responsible for a spectrum of C.difficile infection (CDI) that can be ranged from mild, self-limiting diarrhea to a severe colitis, perforation of colon, pseudomembranous colitis or toxic megacolon and death.The aim of this study was to investigate the antimicrobial susceptibility patterns of C.difficile clinical isolates against antibiotics commonly used for treatment of CDIin hospitalized patients. Methods: During a 12 month study, 75 C.difficile isolates were collected from 390 patients with CDI. All samples were treated with alcohol and yeast extract broth. The treated suspensions were cultured on a selective cycloserine cefoxitin fructose agar (CCFA) supplemented with 5% sheep blood and incubated in anaerobic conditions, at 37 °C for 5 days. In vitro susceptibility of the clinical isolates to 5 antimicrobial agents, including metronidazole, vancomycin, clindamycin, erythromycin and cefotaxime were investigated by agar dilution Methods, according to the CLSI guideline. Results: Metronidazole and vancomycin had good activity against C.difficile isolates with MIC90 of 2 and 1 µg/ml, respectively. Seventy one (94.6%) of the isolates were inhibited by concentrations that did not exceed 2 µg/ml for metronidazole. Resistant to metronidazole observed in 5.3% of the isolates. Out of 43 resistant isolates to erythromycin, 9 (12%) isolates had high-level MIC of more than 64 µg/ml. All strains were resistant to cefotaxime (100%). Sixty seven (89.3%) isolates were resistant to clindamycin (MIC90s>256 µg/ml) and only 6.7% were sensitive to clindamycin. Conclusion: According to our findings, cefotaxim, clindamycin, erythromycin are not effective drugs for treatment of CDI. Although resistant to metronidazole has seen among our isolates but it seems that metronidazole and vancomycin can be effective drugs for treatment of CDI.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :محمد مهدی اصلانی
نویسنده مسئول :مهدی گودرزی
کلیدواژه ها (انگلیسی):Clostridium difficile ; CDI ; MIC
Subjects:QW Microbiology and Immunology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5125
Deposited By: MS Soghra Golmaghani
Deposited On:07 Oct 1392 12:10
Last Modified:20 Dec 1392 09:41

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