مقایسه آزمون PCR و آزمون حلقه ای شیر در تشخیص بروسلا در شیر گاو

شفیعی, بهزاد and احمدی, ملاحت and دستمالچی ساعی, حبیب and کاظمی, جلال (1391) مقایسه آزمون PCR و آزمون حلقه ای شیر در تشخیص بروسلا در شیر گاو. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Comparison of Polymerase Chain Reaction (PCR) and Milk Ring Test (MRT) for Detection of Brucella Spp. in Cattle Milk

English Abstract

Background & Objectives: Brucellosis is an important zoonotic disease in human and animals. The prevalence rate of brucellosis is high all over the world and in most countries including Iran the disease is endemic. Milk , raw milk products and the products of infected animals are a major transmission source of infection to human. Methods: In this research, 60 milk sample collected from suspected cattles in different villages of Kurdestan province . MRT carried out on all samples. DNA extracted for all milk samples directly. In order to detect the Brucella spp. PCR carried out using B4 and B5 primers on all DNAs. Results: Out of 60 milk samples, 17 were positive in MRT and 20 were positive using PCR (PCR products 223 bp). Five samples were just positive in MRT while were negative using PCR. Also 8 samples were just positive in PCR while were not positive by MRT. However the PCR is a sensitive test for detection of bacteria but can not detect all cases which were positive using MRT. Meanwhile some of samples which were positive using PCR were negative in MRT. Conclusion: Based on the results of this research, in order to detect Brucella spp. in milk samples both test (MRT and PCR) recomended to carry simultaneously.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :بهزاد شفیعی
کلیدواژه ها (انگلیسی):Brucella ; Cattle Milk ; MRT ; PCR
Subjects:QW Microbiology and Immunology
WD Nutrition Disease and metabolic diseases
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5182
Deposited By: MS Soghra Golmaghani
Deposited On:15 Oct 1392 12:30
Last Modified:15 Oct 1392 12:30

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