تیمار مزانشیمال استم سل با پپتیدوگلیکان-لیپوپلی ساکارید به عنوان آگونیست TLR2,4 و اندازه گیری آپوپتوز تحت تأثیر زمان در سلول های T

دارابی, الهام and دلیرژ, نوروز and مرشدی, احمد and توکمه چی, امیر and مکاری زاده, آرام (1391) تیمار مزانشیمال استم سل با پپتیدوگلیکان-لیپوپلی ساکارید به عنوان آگونیست TLR2,4 و اندازه گیری آپوپتوز تحت تأثیر زمان در سلول های T. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Treatment of Mesanshimal Stem Cells (MSCs) with Peptidoglycan-LPS as TLR2,4 Agonist Augments Apoptose in Activated T Cells Time Dependently

English Abstract

Background & Objectives: MSCs can interact with cells of both the innate and adaptive immune systems which leads to the modulation of several effector mechanisms based on ability to moderate T-cell proliferation and function. Other reports mainly focused on the role of TLRs in stem cell proliferation and their potential role in disrupting the differentiation capabilities of the stem cells. In the present study invested the coestimolatory effect of peptidoglycan-lipopolysacarid TLR2,4 agonist on apoptosis induction in activated T cells by mouse mesanchymal stem cell (MSCs). Methods: MSCs were isolated from bone-marrow of mice and treated with peptidoglycan-LPS (10ng/ml) as TLR2, 4-agonist for different times (1h and 12h). Treated cells were co-cultured with PHA-activated splenic mononuclear cells (MNCs) for 72h at 37 °C in a humidified 5% CO2 . Apoptosis in activated T cells were then measured using Acridin-Orange/PI staining in flow cytometry. Results: We found that High term exposure (12h) of MSCs to TLR2,4 agonist (peptidoglycan-LPS) con significantly increase of apoptosis in activated T cells in comparison to control group. Conclusion: Our findings suggested that different exposure terms of MSCs to TLR2/4 agonist, differently affected apoptotic activity of MSCs against activated T cells, so, we concluded that TLR2/4 agonist treated MSCs could utilize for moderating the inflammatory reactions in autoimmune disorders.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :الهام دارابی
نویسنده مسئول :احمد مرشدی
کلیدواژه ها (انگلیسی):MSCs ; TLR ; Apoptosis ; T Cell
Subjects:QW Microbiology and Immunology
QZ Pathology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5183
Deposited By: MS Soghra Golmaghani
Deposited On:15 Oct 1392 12:40
Last Modified:15 Oct 1392 12:40

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