بررسي فعاليت ضدباكتريايي MTA, Pro Root MTA ايراني وسمان پرتلند بر روي باكتري هاي انتخابي پاكت پريودنتال در شرايط آزمايشگاهي

بهادر, عباس and علیقلی, مرضیه and شیخ رضایی, محمدسعید and قربان زاده, رقیه (1391) بررسي فعاليت ضدباكتريايي MTA, Pro Root MTA ايراني وسمان پرتلند بر روي باكتري هاي انتخابي پاكت پريودنتال در شرايط آزمايشگاهي. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

Text - Published Version

Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


Invitro Study of Antibacterial Activity of Pro Root MTA, Iranian MTA and Portland Cement on Selective Periodontal Pocket’s Bacteria

English Abstract

Background & Objectives: Microorganisms are the main etiological factors in pulpitis, apical periodontitis and endodontic disease. As a result, their elimination during RCT by instrumentation, irrigation and intracanal medication is essential. However, even after these procedures, bacteria may still be found within the root canal system. One of the reasons for endodontic failure is inadequate obturation of the canal spaces. The presence of communication between the internal and external environments, such as a periodontal pocket, or remnants of residual bacteria due to inadequate cleaning and shaping can cause failure of the root treated tooth; therefore the antimicrobial activity of filling materials such as mineral trioxide aggregate (MTA), may play a vital role in the success rates of endodontic treatment. The purpose of this study was to evaluate the antibacterial effect of Pro Root MTA, Iranian MTA and Portland cement. Methods: Broth dilution test and agar diffusion test against 3 of periodontal pocket microorganisms was used. Actinobacillus actinomycetemcomitans (Aa Bacilli), F. nucleatum and P. gingivalis were tested against Pro Root MTA (PRMTA), Iranian MTA (IMTA) and Portland Cement (PC) adjusted to 50mg/ml and clinical concentration (70mg/25µl). The agar diffusion Methods on Blood Agar with Hemin and Vitamin K was employed. The microorganisms were seeded by speader. Wells were made by removing agar at equidistant points. PRMTA, IMTA and PC were placed into the wells immediatelly after manipulation. The plates incubated in the anaerobic atmosphere at 37 °C for 72 h and the zones of inhibition were measured. In the broth dilution test Contact times used were 0h, 1h, 3h, 48 h, and 72h. Results: In the agar diffusion test PRMTA, and IMTA against Aa Bacilli and F. nucleatum showed zones of inhibition whereas PC had no antimicrobial activity against F. nucleatum. PRMTA, IMTA and PC did not demonstrate any antimicrobial inhibition against P. gingivalis. Analyzing the antimicrobial activity of PRMTA, IMTA and PC showed a statistically significance difference (p < 0.05) between PRMTA, IMTA and PC. IMTA showed the largest zone of inhibition (2.9 mm) against Aa Bacilli. There was no difference in the zones of inhibition between the 48- and 72-h time periods. According to conventional standards, the borderline of antimicrobial effect was determined at 0.1% viable cells (99.9% reduction of CFU). In the broth dilution test results showed that the 50mg/ml and clinical concentration of PRMTA and IMTA was effective in the tested bacteria after 3h of contact. The 50mg/ml and clinical concentration of IMTA and PRMTA demonstrated antimicrobial activity only for Aa bacillai (p < 0.05) but they delayed or limited growth of F. nucleatum and P. gingivalis. For 50mg/ml and clinical concentration PC, only Aa bacillai was inhibited. No antimicrobial activity was detected for PC. After 3h of contact clinical concentration of IMTA and PRMTA showed greater effect than the 50mg/ml. Conclusion: The inhibitory effect of anti-bacterial effect of IMTA against Actinobacillus actinomycetemcomitans, F. nucleatum and P. gingivalis is superior than PRMTA and Portland cement

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :عباس بهادر
کلیدواژه ها (انگلیسی):Antimicrobial Activity ; Pro Root MTA (Mineral Trioxide Aggregate) , Iranian MTA; Portland Cement ; A. Actinomycetemcomitans; F. Nucleatum; P. gingivalis
Subjects:QW Microbiology and Immunology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5184
Deposited By: MS Soghra Golmaghani
Deposited On:15 Oct 1392 12:46
Last Modified:15 Oct 1392 12:46

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