بررسی مولکولی مهمترین فاکتورهای حدت همزمان با تیپ بندی کپسولی پاستورلا مالتوسیدای جدا شده از گوسفند در استان فارس

Title

Molecular Evaluation of Major Virulence Genes Simultaneous with Capsular Typing of Pasteurella multocida Isolated From Sheep in Fars Province

English Abstract

Background & Objectives: Pasreurella multocida causes a wide range of important diseases in domestic animals, being responsible for pneumonia in cattle and sheep.The aim of this study was to learn more about the virulence of P. multocida isolated from sheep. Methods: In this purpose we designed a multiplex PCR assay for simultaneous detection of major important virulence genes including ptfA, hgbA, toxA, ompH, along with capsular typing. Thirty one isolates of P.multocida isolated from diseased sheep were evaluated. Results: Capsular typing of isolates by PCR demonstrated three capsular types A , D, with prevalence of 83.8% and 9.6%, respectively. The results also showed that 30 (96.7%) , 28 (90.3%) , 21(67.7%) and 24(77.4%)of isolates were positive for precense of toxA, hgbA, ptfA and ompH genes respectively. Conclusion: P. multocida capsular type A was the most common in the isolates. The remarkable high prevalence of toxA and hgbA among diseased sheep may imply to important role of these genes in epidemiology and virulence of P.multocida. Furthermore the high prevalence of P. multocida typeA harbor toxA gene found is noticeable and attribute to its important role in respiratory infection.

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