title

بررسي اثر موتاسيون بر توليد سلولاز در سويه هاي تريکودرما

سجادی, طاهره and آذین, مهرداد and مظاهری اسدی, مهناز (1391) بررسي اثر موتاسيون بر توليد سلولاز در سويه هاي تريکودرما. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Title

Production of Cellulases by Trichoderma Spp. Mutants

English Abstract

Background & Objectives: Cellulose is the most abundant biopolymer in nature. Cellulases, which are mainly three types: endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC3.2.1.91) and beta-glucosidases (EC3.2.1.21) catalyze the hydrolysis of cellulose. Enzymatic hydrolysis of cellulosic biomass are used in different fields, such as production of fuel ethanol from released glucose.Trichoderma species are known as the most potent producers of cellulases. Selection of the Trichoderma mutant strains showing increased cellulase production. Methods: Seven Trichoderma strains were obtained from Persian Type Culture Collection and were cultured on PDA medium. In order to select the best fungi, cellulase activity of native fungi was assayed. Carboxymethyl cellulose sodium (CMC-Na), Avicel and cellobiose were used for endoglucanase, cellobiohydrolase (exoglucanase) and cellobiase (beta-glucosidase) assays, respectively. A sample of 108 conidiospores/ml was treated by 0.2M Sodium Nitrite for 53 minutes at 30°C,while stirred at 150 rev.min-1, by which methods, 99.99% mortality was observed. Mutated spores were plated on the Mandel’smineral salts medium and their cellulase activities were judged on the basis of hollow zone diameter surrounding the colonies (H/C). Selected mutants were screened in production medium for their cellulase activity. Results: Trichoderma parceramosum (PTCC5140) was selected as the best wild type strain with the highest cellulase activity. After mutagenesis, among 70 obtained mutants,10 isolates with larger clearing zones were assessed in shake flask cultures.The mutant strain T2 and T5 produced 2.5 and 2.27-fold endoglucanase, respectively. Cellobiohydrolase activity of mutant T10 was 1.3-fold in the comparison with wild type. T9 and T10 produced 3.7 and 4.15 times more beta-glucosidasethan the parent strain. Conclusion: Cellulase produced by both parent and mutant strains were evaluated for their efficiency of bioconversion or saccharification. Mutant T10 showed improvement of cellulase production, mainly in cellobiohydrolase and beta-glucosidase activity could be a potential candidate for the bioconversion process.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :طاهره سجادی
کلیدواژه ها (انگلیسی):Cellulase ; Mutant ; Nitrous Acid ; Trichoderma parceramosum
Subjects:QW Microbiology and Immunology
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5190
Deposited By: MS Soghra Golmaghani
Deposited On:16 Oct 1392 09:30
Last Modified:16 Oct 1392 09:30

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