اهمیت تغییرات در کدون 305 ژن katG مایکوباکتریوم توبرکلوزیس در بروز فنوتیپ مقاومت به ایزونیازید

لیوانی, صدیقه and نعمتی شجاعی, الناز and جاوید, سیده ناعمه and رفیعی, سهیل and قائمی, عزت اله (1391) اهمیت تغییرات در کدون 305 ژن katG مایکوباکتریوم توبرکلوزیس در بروز فنوتیپ مقاومت به ایزونیازید. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


The Significance of Mutation on Codon 305 of KatG on Emerging of Resistance Phenotype of M. Tuberculosis

English Abstract

Backgrund & Objectives: The aim of this study was to evaluate the role of mutations in codon 305 in katG gene in isoniazid resistant phenotype. Methods: A region containing codon 305 of katG gene was amplified by PCR. GGC to GCC mutation was detected by BanI endonuclease. The results were compared with the results of drug susceptibility testing with Mycobacteria growth indicator tube (MGIT). Results: 26 isolates were resistant to isoniazid by MGIT. Of these, 4 isolates had mutations in codon 305. Specificity of this endonuclease to identify resistant cases, was estimated to be 98%. Conclusion: BanI endonuclease was able to detect 15% of cases resistant to isoniazid (4 of 26 resistant). BanI endonuclease has a high specificity to identify true sensitive strains.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :صدیقه لیوانی
کلیدواژه ها (انگلیسی):Mycobacterium tuberclus ; Isolates
Subjects:QW Microbiology and Immunology
QZ Pathology
WC Communicable Diseases
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5191
Deposited By: MS Soghra Golmaghani
Deposited On:16 Oct 1392 09:37
Last Modified:16 Oct 1392 09:37

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