ژنوتایپینگ استافیلوکک های کواگولازمنفی جدا شده از نمونه های کلینیکی بیمارستان شهید محمدی بندرعباس با سکانس نمودن ژن tuf

اژدری, افشین ، جوادپور, صدیقه ، انصاری, مریم ، تمدنی, سعید (1391) ژنوتایپینگ استافیلوکک های کواگولازمنفی جدا شده از نمونه های کلینیکی بیمارستان شهید محمدی بندرعباس با سکانس نمودن ژن tuf. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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آدرس اینترنتی رسمی : http://congress.arums.ac.ir/index.php/IICM/5/sched...

عنوان انگليسي

Genotyping of Clinical Isolates of Coagulase-Negative Staphylococci Species by PCR-Sequencing of Tuf Gene

خلاصه انگلیسی

Background & Objectives: Over the last two decades CONs have emerged as opportunistic pathogens, especially among immunocompromised hosts and patients with implanted biomatherials. The increasing incidence of these micro-organisms in hospital- acquired infections, necessitates the need for an accurate identification of staphylococcus isolates at the species level. Since tuf gene is known as the most accurate for specifying CONs, we aimed to identify species of 50 consecutive clinical CONs isolates by phenotypic characteristics and tuf gene sequencing. Methods: A total of 50 CONs was isolated from various clinical specimens of hospitalized patients in Shahid Mohammadi hospital, Bandar-Abbass. Phenotyping was carried out by differential - biochemical tests. Genotyping was performed by sequencing of tuf gene, followed by blast and construction of phylogenetic tree. Results: The clinical isolates consisted of 25(50%) S. epidermidis, 22(44%) S. saprophyticus and 3(6%) S. hemolyticus. S. epidermidis and S. saprophyticus strains were mostly isolated from blood and urine cultures, respectively. Vancomycin was found to be the most effective antibiotic followed by cefalexin and ofloxacin. Blast searches and phylogenetic tree inferred from the neighbor-joining methods of 8 isolates revealed that 5 isolates had a tuf sequence 100 % identical to tuf gene of S. epidermidis strain SeMCV45, and the tuf gene of 3 isolates was 99% similar to that of S. haemolyticus strain ShlMCV14. Conclusion: CONS are involved in a wide range of nosocomial infections. PCR and sequencing of the tuf gene is a reliable and valuable approach for the genotyping and identification of CONS species in epidemiological studies.

نوع سند :موضوع کنفرانس یا کارگاه (پوستر )
زبان سند : انگلیسی
نویسنده مسئول :افشین اژدری
کلید واژه ها (انگلیسی):Coagulase Negative Staphylococci; Tuf Gene ; Clinical Samples
موضوعات :QW میکروب شناسی و ایمنی شناسی
WX بیمارستانها و دیگر مراکز درمانی
بخش های دانشگاهی :معاونت تحقیقات و فناوری > مديريت تحقیقات و فناوری و اطلاع رساني > مدیریت همایش ها و کنگره های دانشگاه
کد شناسایی :5202
ارائه شده توسط : خانم صغری گلمغانی
ارائه شده در تاریخ :16 دی 1392 11:00
آخرین تغییر :16 دی 1392 11:00

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