معرفی روشي ساده، سریع و ارزان جهت استخراج DNA از گونه هاي نوکاردیا با استفاده از پودر ماشین لباسشوئی

فامیلی, عسل بانو and کچویی, رضا and میرنژاد, رضا and امیر مظفری, نور and ایمانی فولادی, عباسعلی (1391) معرفی روشي ساده، سریع و ارزان جهت استخراج DNA از گونه هاي نوکاردیا با استفاده از پودر ماشین لباسشوئی. در: The 13th Iranian & The Second International Congress of Microbiology, July 14 – 16, 2012, Ardabil - Iran.

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Official URL: http://congress.arums.ac.ir/index.php/IICM/5/sched...


A Simple, Rapid and Inexpensive Methods for Isolation of DNA from Nocardia Spp. Using Laundry Detergent

English Abstract

Background & Objectives: Rapid diagnosis of Nocardiosis and early treatment, is necessary to use molecular Methods that Requires DNA extraction of nocardia. Since different methods for extracting DNA from microorganisms are time consuming and laborious, and the use of chemical compounds separately in routine methods of DNA extraction is not economical. In order to minimize time and cost required for DNA extracted from nocardia is the methods of DNA extraction with five enzyme washing machine powder is used. This methods without harmful effects on the genome may affect the bacterial cell wall. Introduced a quick and inexpensive methods for DNA extraction from nocardia. Methods: DNA extraction from five enzyme washing machine powder taj brand (containing amylase, protease, lipase, mannanase and cellulase), and to review and confirm the presence of DNA from agarose gel and specific primers for polymerase chain reaction is used. Results: DNA extraction and analysis of results obtained by electrophoresis of PCR products showed that in this way DNA is extracted. Conclusion: According to the results obtained using the washing machine powder for DNA extraction can be used as a rapid and inexpensive alternative than routine procedures.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :عسل بانو فامیلی
نویسنده مسئول :رضا کچویی
کلیدواژه ها (انگلیسی):Nocardia ; Genome ; Extraction
Subjects:QW Microbiology and Immunology
WA Public Health
WA Public Health
Divisions:Vice Chancellor for Research and Technology > Deputy for Research and Technology management and Medical Information > University of Management Conferences and Congresses
ID Code:5212
Deposited By: MS Soghra Golmaghani
Deposited On:16 Oct 1392 11:47
Last Modified:31 Mar 1393 08:49

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