اثر ترکيبات روغني و آلکاني بر روي القاء پروموتور POX2 در مخمر ياروويا ليپوليتيکا

Title

Effect of Oleic and Alkane Compounds on POX2 Promoter Induction in Yarrowia lipolytica Yeast

English Abstract

Background & Objectives: Several regulated promoters are now available for heterologous genes expression in Yarrowia lipolytica. POX2 codes for the long-chain acyl-coenzyme A oxidase, which is repressed by glucose and induced by fatty acids and oils. In this study, effect of oleic and alkane compounds is investigated on POX2 promoter induction in Y. lipolytica. Methods: The recombinant Y. lipolytica JMY768 strain with a pPOX2-lacZ reporter gene cultured in the media containing different concentrations of olive oil, oleic acid and methyl oleate as oleic compounds, C10 - C18 alkanes and tributyrin. The β-galactosidase activity and growth rate were investigated. Results: Theoleic acid 0.5%, C16 1% and olive oil 0.5% had good induction on POX2 promoter. Furthermore, the yeast recombinant strain has good growth on media containing oleic acid, C16 alkane and olive oil. Conclusion: The XPR2, ICL1, POX2 and POT1 regulated promoters are available. The oleic acid, C16 alkane and olive oil could be use as cheap substrates for high-level production of valuable products and heterologous proteins by Y. lipolytica recombinant strains with POX2 promoter.

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