بررسی کمی فعالیت آنزیم تلومراز به روش SYBR Green RT-TRAP در بیماران مبتلا به سرطانهای معده و مری در استان اردبیل

حسینی اصل, سید سعید and رحمانی, سید علی and فیروز, نوروزی (1392) بررسی کمی فعالیت آنزیم تلومراز به روش SYBR Green RT-TRAP در بیماران مبتلا به سرطانهای معده و مری در استان اردبیل. Masters (پایان نامه) دانشگاه آزاد اسلامی واحد اهر.

[img] Text - final reserch project report
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Text - final reserch project report


SYBR Green based RT-TRAP assay of the Telomerase activity in patients affected with gastric or esophageal cancers in Ardabil province

English Abstract

Background and objective : astric cancer is the fourth most common cancer and second leading cause of cancer related death in the world with under 5-year survival . Original Article Gastric and esophageal cancers are the most frequent causes of cancer-related deaths in Iran with the highest rates reported from Ardebil.The survival incidence for patients affected with these cancers in ardebil is under 2 years. Telomerase is a cellular reverse transcriptase (molecular motor) that adds new DNA onto the telomeres that are located at the ends of chromosomes. Telomerase is repressed in most human somatic cells, but reactivated in more than 80% of all human cancers. Materials and methods : 30 patient with Gastric and Esophageal Cancer that their disease confirmed histologically from ardabil emam khomaine hospital were studied in this project. Then telomerase protein was extracted by chaps buffer and Protein concentration of extracts was determined with the Protein Assay (Bio-Rad), Then telomerase activity assaied with both TRAP Assay and SYBR Green RT-TRAP Assay. Results : 90% of Patients Affected with Gastric and Esophageal Cancer had telomerase activity. Discussion : telomerase is potent biomarker for diagnosis and cancer therapotic.

Item Type:Thesis (Masters)
زبان سند : فارسی
استاد راهنما :سید سعید حسینی اصل
استاد مشاور :سید علی رحمانی
نگارنده :نوروزی فیروز
کلیدواژه ها (انگلیسی):Gastric and Esophageal Cancer , Telomerase , TRAP Assay , SYBR Green RT-TRAP Assay
Subjects:QY Clinical Pathology
WI Digestive System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Genetics
ID Code:5259
Deposited By: Dr S Saied Hosseini-Asl
Deposited On:18 Oct 1392 10:50
Last Modified:07 Jul 1393 05:28

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