ارزنلو, محسن and رضایی, عباس and شاهرخی, نادر and زواران حسینی, احمد and یاسودا, یوکو and توچیکوبو, کونیو and آهنگرزاده رضایی, محمد (1384) بیان زیرواحد B سم کلرادر ساکارومیسس سرویزیه. Annals of Microbiology ــ 55 (2). pp. 145-155. شاپا 1590-4261
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Official URL: http://www.annmicro.unimi.it/index.htm
Title
Expression of cholera toxin B subunit in Saccharomyces cerevisiae
English Abstract
Cholera toxin, secreted by Vibrio cholerae, consists of A and B subunits. Cholera toxin B subunit (CTB) is used in many scientific research. It has already been expressed in several bacterial and plant systems. In order to express CTB protein in Saccharomyces cerevisiae, the expression plasmid pCTB83 was constructed by inserting ctxB gene in pYES2 shuttle vector. The new construct was transferred into S. cerevisiae cells and the ctxB gene was induced with 2% galactose. SDS-PAGE analysis showed the presence of CTB in yeast lysate and immunoblotting analysis of yeast total soluble protein indicated that the yeast-derived CTB protein was antigenically indistinguishable from bacterial CTB protein. Quantitative ELISA showed that the maximum amount of CTB protein expressed in yeast was approximately 1.8% of total soluble protein. CTB is a subunit vaccine candidate against cholera. Since the whole recombinant yeast has been introduced as a new vaccine formulation, expression of ctxB in S. cerevisiae may offer an effective and inexpensive strategy to protect people against cholera in high-risk areas.
| Item Type: | Article |
|---|---|
| زبان سند : | انگلیسی |
| نویسنده اول : | محسن ارزنلو |
| نویسنده مسئول : | عباس رضایی |
| Additional Information: | Impact factor: 0.532 Abstracted/Indexed in::1- Science Citation Index Expanded 2-BIOSIS,SCOPUS, 3- AGRICOLA, Biological Abstracts, Biotechnology Citation Index, CAB Abstracts, CAB International, Chemical Abstracts Service (CAS), Food Science and Technology Abstracts, Global Health, Google Scholar, IBIDS, Journal Citation Reports/Science Edition, OCLC, , Sociedad Iberoamericana de Informacion Cientifica (SIIC) Databases, Summon by Serial Solutions |
| Uncontrolled Keywords: | ساکارومیسس سرویزیه ، زیرواحد B سم کلرا ، ویبریوکلرا |
| کلیدواژه ها (انگلیسی): | Saccharomyces cerevisiae , CTB , gen expression , Vibrio cholera |
| Subjects: | QW Microbiology and Immunology |
| Divisions: | Faculty of Medicine > Department of Basic Sciences > Department of Microbiology |
| ID Code: | 527 |
| Deposited By: | Dr Mohsen Arzanlou |
| Deposited On: | 23 Sep 1388 07:15 |
| Last Modified: | 24 Nov 1391 08:32 |
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