شناسایی و تعیین هویت گونه های کریپتوسپوریدیوم در نمونه های آب بدست آمده از یک رودخانه در شهر اردبیل، شمال غرب ایران

محمدی قلعه بین, بهنام and فلاح, اسماعیل and اصغرزاده, محمد and کاظمی, عبدالحسن and ارزنلو, محسن (1386) شناسایی و تعیین هویت گونه های کریپتوسپوریدیوم در نمونه های آب بدست آمده از یک رودخانه در شهر اردبیل، شمال غرب ایران. Research Journal of Biological Sciences ــ 2 (4). pp. 498-502. شاپا 1815-8846

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Detection and Identification of Cryptosporidium species in Water Samples from a River in Ardabil City, Northwestern Iran

English Abstract

Cryptosporidium is an opportunistic parasite typically associated with large waterborne outbreaks. Surface waters contaminated with human and animal feces serve as main source for epidemic spread of Cryptosporidium parasites. In this study, we used a small-subunit rRNA-based PCR-Restriction Fragment Length Polymorphism (RFLP) technique to determine the prevalence and to characterize human-infective species of Cryptosporidium parasites in water samples collected from a stream in Ardabil city in Iran. Among 30 samples examined, 11 samples showed positive results. Restriction pattern analysis showed C. andersony as the most common species with 7 cases; followed by C. parvum, bovine genotype, with 3 cases and C. suis with 1 case. The results indicated that PCR-RFLP technique provides an applicable and feasible method for detection and identification of Cryptosporidium oocysts in environmental water samples. The results, furthermore, demonstrated that wildlife is the major source of Cryptosporidium oocysts in surface water resources in the study region.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :بهنام محمدی قلعه بین
نویسنده مسئول :محسن ارزنلو
Additional Information:index abstract: 3- DOAJ, IndexCopernicus , Zoological Record
Uncontrolled Keywords:گونه های کریپتوسپوریدیوم ، PCR-RFLP ، آب ، تشخیص ، تعیین هویت ، ایران
کلیدواژه ها (انگلیسی):Cryptosporidium species , PCR-RFLP , water , detection , identification , Iran
Subjects:QX Parasitology
QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:530
Deposited By: Dr Mohsen Arzanlou
Deposited On:23 Sep 1388 06:26
Last Modified:10 Jul 1393 07:42

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