آزمایش اوره آز مستقیم و رنگ آمیزی آکریدین اورنج بر روی محیط های کشت BACTECجهت تشخیص سریع احتمالی بروسلوز

مالک نژاد, پرویز and بنکدار هاشمی, فرهاد and فتح اله زاده, بهرام and جعفری, سیروس and پیری دوگاهه, هادی (1384) آزمایش اوره آز مستقیم و رنگ آمیزی آکریدین اورنج بر روی محیط های کشت BACTECجهت تشخیص سریع احتمالی بروسلوز. Iranian J Publ Health ــ 34 (3). pp. 52-55. شاپا 2251-6085

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Direct Urease Test and Acridine Orange Staining on Bactec Blood Culture for Rapid Presumptive Diagnosis of Brucellosis

English Abstract

Brucellosis is one of the most common zoonotic diseases in Iran and human brucellosis is endemic in all parts of the country. Growth of Brucella is slow and blood culture of these bacteria by use of classical methods is time-consuming. Furthermore, in endemic area culture is required for definitive diagnosis. In the present study, direct urease test and acridine orange staining were tried on the BACTEC blood culture broths for early presumptive identification of Brucella growth. Blood cultures were attempted in 102 seropositive patients. In the forty one blood cultures positive for Brucella, coccobacilli were seen in broth smears stained with acridine orange stain, and also were urease test positive, thus providing presumptive identification of Brucella growth. Urease test was negative and bacteria were not seen in the broth smears of the remaining 61 broths negative for Brucella growth. Because of simplicity, reliability and reproducibility, these tests can be routinely incorporated in the laboratory for diagnosis of brucellosis.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :پرویز مالک نژاد
نویسنده مسئول :هادی پیری دوگاهه
Additional Information:Impact Factor : 0.378 his Journal is indexed and abstracted in: Science Citation Index Expanded (SCIE) by Thompson/ISI USA, EMBASE, Chemical Abstracts, SCOPUS, ASCI, Biological Ab­stracts, IMEMR, CABI Publishing, Index Copernicus, Medscape, VCU Library, Extra MED, DOAJ, Ovid Medline (R), Magiran, SID, Iran Medex and Islamic World Science Citation Center (ISC), EBSCO, CINAHL
کلیدواژه ها (انگلیسی):Brucella spp , Brucellosis , Urease test , Acridine orange , BACTEC blood culture , Iran
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:559
Deposited By: Dr Hadi Peeridogaheh
Deposited On:02 Oct 1388 11:24
Last Modified:07 Jul 1393 06:33

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