title

ارزیابی جفت پرایمر های متفاوت برای تشخیص سریع سل

فتح اله زاده, بهرام and مالک نژاد, پرویز and بهادر, عباس and پیری دوگاهه, هادی and علیخانی, محمد یوسف (1386) ارزیابی جفت پرایمر های متفاوت برای تشخیص سریع سل. Pakistan journal of biological sciences ــ 10 (1). pp. 107-111. شاپا 1028-8880

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Title

Evaluation of Diffrent Primer Sets for the Rapid Diagnosis of Tuberculosis

English Abstract

Pleural fluid samples from patients with exudative effusion who were diagnosed with tuberculous pleuritis are examined using a new designed primer set based on IS1081 gene (IS1081-PCR) and rpoB-PCR. The PCR results are compared with the results of the sample cultures, using Loewenstein-Jensen (LJ) medium and Ziehl-Neelsen (ZN) staining. Of 78 cases that were confirmed as tuberculous pleuritis by histopathology, supported by sputum culture, biochemical markers (adenosine deaminase, gamma interferon and tumor necrosis factor), radiographic and clinical data, 61 (78.2%) were positive by IS1081-PCR, 43 (55.1%) by rpoB-PCR, 17 (21.7%) by culture and 3 (3.8%) by ZN stain. When IS1081-PCR test results were compared with the confirmed culture, the sensitivity, specificity, positive predictive value and negative predictive value for the IS1081-PCR were 94.1, 55.7, 37.2 and 97.1%, respectively. The corresponding values for the rpoB-PCR were 94.1, 26.2, 26.2 and 94.1%, respectively. When tests results were compare with the confirmed radiographic, histopathology, biochemical markers and clinical diagnosis of tuberculous pleuritis, the IS1081-PCR assay is more sensitive, specific and reliable than both rpoB-PCR assay and culture.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :بهرام فتح اله زاده
نویسنده مسئول :پرویز مالک نژاد
نویسنده :هادی پیری دوگاهه
Additional Information: Indexed in: ASCI-Database, CABI , Cambridge Scientific Abstract , Chemical Abstract Services , Directory of Open Access Journals , FSTA ,Google Scholar , SCIMAGO ,SCOPUS
کلیدواژه ها (انگلیسی):Tuberculosis plueritis , PCR , Ziehl Neelsen , Loewenstein-Jensen medium
Subjects:QW Microbiology and Immunology
WA Public Health
WA Public Health
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:563
Deposited By: Dr Hadi Peeridogaheh
Deposited On:13 Oct 1388 05:03
Last Modified:18 Nov 1391 14:15

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