کشت و تمایز سلولهای بنیادی و پیش ساز عصبی بر روی نانوداربست پلیمری پلی-ال-لاکتیک اسید

میری, وحیده and گل محمدی, محمد قاسم and اسدی, اسداله and سقا, محسن (1392) کشت و تمایز سلولهای بنیادی و پیش ساز عصبی بر روی نانوداربست پلیمری پلی-ال-لاکتیک اسید. در: Royan International Tween Congress-9th Congress on stem cell biology and technology, September 4-6, 2013, Iran - Tehran.

Text - Published Version


Culture and differentiation of mouse neural stem and progenitor cells in a polymeric nano-scaffold Poly-L Lactic Acid

English Abstract

Objectives: The selection of a good quality scaffold is an essential strategy for tissue engineering. Ideally, the scaffold should be a functional and structural biomimetic of the native extracellular matrix and support multiple tissue morphogenesis. Neural stem/progenitor cells (NSCs) are intrinsically capable of differentiating into different neural cell types within the nervous system, offering prospects for NSC-based cell therapies to treat neurodegenerative diseases. In this study, an attempt was made to develop porous polymeric Nano-fiber scaffold using a biodegradable Poly (l-lactic acid) (PLLA) for in vitro culture of neural stem and progenitor cells. Materials & methods: To fabricate Nano-fiber, we used electrospinning techniques. The physico-chemical properties of the scaffold were fully characterized by using scanning electron microscopy. NSCs were isolated from the subventricular zone of the adult mouse brain and cultured in the scaffold and then the viability, proliferation and differentiation of these cells determined via both of Immunostaining and MTT assay. Scanning Electron Microscopy (SEM) was also used to observe the morphology of the cells cultured on PLLA fibrous scaffolds. Results: DAPI and Immunostaining revealed the neuronal differentiation and adhering of cells on the surface of the electrospun scaffolds. Blue stained cells were found in Nano-fiber indicating that the scaffold had provided basic cell attachment. The MTT assay results showed that the absorption in 570 nm increased during 2 to 5 days. The morphology of differentiated cells was evaluated by SEM. Conclusion: Nano-Scaffold PLLA due to the biocompatible properties is an appropriate structure for proliferation, differentiation and normal growth of the neural stem and progenitor cells.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :وحیده میری
نویسنده مسئول :محمد قاسم گل محمدی
نویسنده :اسداله اسدی
نویسنده :محسن سقا
کلیدواژه ها (انگلیسی):Tissue engineering, Nano-fiber scaffold, Neural stem cells
Subjects:QS Human Anatomy
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:5663
Deposited By: Dr Mohammad Gasem Golmohammani
Deposited On:20 Feb 1393 09:29
Last Modified:20 Feb 1393 09:29

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