مقایسه روشهای تشخیصی در بیماران بروسلوزی بستری شده در بیمارستان در ایران

امیرزرگر, علی اکبر and حسیبی, مهرداد and مالک نژاد, پرویز and پیری دوگاهه, هادی and جعفری, سیروس and حاجيا, مسعود (1388) مقایسه روشهای تشخیصی در بیماران بروسلوزی بستری شده در بیمارستان در ایران. Infectious Diseases in Clinical Practice ــ 17 (4). pp. 239-242. شاپا 1056-9103

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Comparison of Diagnostic Methods in Hospitalized Patients With Brucellosis in Iran

English Abstract

Diagnosis of brucellosis is based on clinical symptoms and positive laboratory results. Because specificity of the serology tests is not satisfactory and sensitivity of the culture is low, polymerase chain reaction (PCR) can be an alternative method in making the final decision in some doubtful cases. In this study, results of standard tube agglutination, specific immunoglobulin (Ig) G and IgM by enzyme-linked immunosorbent assay, and culture were compared with PCR results in 45 patients with brucellosis referred to the Imam Khomeini hospital, Tehran, Iran. The AQ3 BACTEC system was applied to isolate the organisms from blood samples, which were then identified by the urease, oxidase, H2S production, and catalase tests. Primer pair was applied to amplify a 223-base-pair fragment of the bcsp31 gene. Analysis of the results revealed that IgG test, IgM test, standard tube agglutination, culture, and PCR results were positive in 97.77%, 40%, 71.11%, 31.11%, and 48.88% of the cases, respectively.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :علی اکبر امیرزرگر
نویسنده :هادی پیری دوگاهه
نویسنده مسئول :مسعود حاجيا
Additional Information:Indexed/abstracted in: 2-EMBASE, SCOPUS, Current Contents/Clinical Medicine
کلیدواژه ها (انگلیسی):brucellosis , Diagnosti method , Iran
Subjects:WB Practice of Medicine
QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:580
Deposited By: Dr Hadi Peeridogaheh
Deposited On:14 Oct 1388 07:03
Last Modified:07 Jul 1393 06:35

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