تعیین مولکولی جهش مدیترانه (G6PD) در شمال غرب ایران

ولیزاده, مهدی and عنصری, حبیب and فتحی, افشین and جبارپوربنیادی, مرتضی and رضامند, عظیم and امانی, فیروز (1393) تعیین مولکولی جهش مدیترانه (G6PD) در شمال غرب ایران. Journal of Physiology and Pharmacology Advances ــ 4 (7). pp. 389-395. شاپا 2251-7693

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Official URL: http://www.scopemed.org/?mno=165180


Molecular Identification of Mediterranean Mutation in Patients with Deficiency of Glucose-6-Phosphate Dehydrogenase (G6pd) in North West of Iran

English Abstract

Glucose-6-phosphate dehydrogenase (G6PD) is a highly polymorphic enzyme that is encoded by human X-linked gene.G6PD deficiency is the most common enzymopathy in human with approximately 400 million people affected worldwide. According to the previous studies, Mediterranean mutation (C563T) is one of the most common G6PD enzyme mutations in Iranian population with a prevalence range of %63 to %86/4.This study was conducted in order to determine Mediterranean mutation, through molecular method ,in the population of the North-west of Iran. In this study, by Rapid Genomic DNA Extraction (RGDE) method, from 77 blood samples of unrelated male and female patients with genetic deficiency of G6PD, DNA was extracted and after digestion by MboII enzymes, in order to search for Mediterranean mutation, they were analyzed by means of PCR-RFLP and sequencing methods.Of the 77 patients, 48 patients (37 male, 11 female) had a Mediterranean mutation frequency of 62/3 %. 29 patients (37/7%) had mutations other than Mediterranean type.This study revealed that G6PD is the most common mutation in Iranian North-west population. Further studies are recommended to identify the mutation type of other varieties.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مهدی ولیزاده
نویسنده مسئول :حبیب عنصری
نویسنده :افشین فتحی
نویسنده :فیروز امانی
Additional Information:Indexing: DOAJ, Chemical Abstract Service (CAS), Google Scholar, Open J-Gate, ScopeMed, Scientific Publications Index, Scientific Resources Database, Recent Science Index, Scholarly Journals Index, Directory of Academic Resources, Elite Scientific Journals Archive, Current Index to Scholarly Journals, Digital Journals Database, Academic Papers Database, Contemporary Research Index, Index Copernicus.
کلیدواژه ها (انگلیسی):Glucose-6-phosphate dehydrogenase, mediterranean mutation, north- west of Iran
Subjects:WA Public Health > WA. 900 Vital Statistics
WA Public Health > WA. 900 Vital Statistics

QU Biochemistry
QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Pediatrics , Community Medicine
Faculty of Medicine > Department of Basic Sciences > Department of Vital Statistics
ID Code:5886
Deposited By: Dr Firouz Amani
Deposited On:01 Jun 1393 04:30
Last Modified:01 Jun 1393 04:30

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