تمایز عصبی سلولهای بنیادی CD34 مثبت فولیکول مو با استفاده از سه روش تمایز نورونی

نجف زاده, نوروز and سقا, محسن and حیدری تجدد, شیرین and گل محمدی, محمد قاسم and مساحی اسکویی, نسیم and دلداده مقدم, مریم (1393) تمایز عصبی سلولهای بنیادی CD34 مثبت فولیکول مو با استفاده از سه روش تمایز نورونی. In Vitro Cellular & Developmental Biology - Animal ــ 51 (2). pp. 192-203. شاپا 1071-2690

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Official URL: http://link.springer.com/article/10.1007%2Fs11626-...


In vitro neural differentiation of CD34 + stem cell populations in hair follicles by three different neural induction protocols

English Abstract

Differentiation of hair follicle stem cells (HFSCs) into neurons and glial cells represents a promising cell-based therapy for neurodegenerative diseases. The hair follicle bulge area is reported as a putative source of new stem cell population for many years. In vitro studies have implicated neural differentiation of HFSCs. Here, we report the identification and purification of CD34 + cells from hair follicle by magnetic activated cell sorting (MACS). We next determined the cytotoxic effects of all-trans retinoic acid (RA) by using cell viability assays. Moreover, the neural differentiation potential of CD34 + cells was evaluated in the presence of RA, serum-free condition, and neural differentiation medium (NDM) treatments by using immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). Our results showed that the isolated CD34 + stem cells were 12% of the total cells in the bulge area, and the neural cells derived from the stem cells expressed nestin, microtubule-associated protein 2 (MAP2), and glial fibrillary acidic protein (GFAP). Interestingly, all the neural induction media supported neuronal differentiation most effectively, but treatment with serum-free medium significantly increased the number of GFAP-positive glial cells. Moreover, increasing RA concentration (≥10 μM) leads to increased cell death in the cells, but a lower concentration of RA (1 μM) treatment results in a decrease in CD34-expressing stem cells. These findings show an instructive neuronal effect of three neural induction media in HFSCs, indicating the important role of this induction media in the specification of the stem cells toward a neural phenotype.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :نوروز نجف زاده
نویسنده :محسن سقا
نویسنده :شیرین حیدری تجدد
نویسنده :محمد قاسم گل محمدی
نویسنده :نسیم مساحی اسکویی
نویسنده :مریم دلداده مقدم
Additional Information:Impact Factor(2014)1.145 Indexed in: ISI, PubMed/Medline, Scopus
کلیدواژه ها (انگلیسی):CD34 , Isolation , Hair follicle stem cells , All-trans retinoic acid , Neuronal cell , Glial cell
Subjects:QS Human Anatomy
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:5987
Deposited By: Dr Nowruz Najafzadeh
Deposited On:19 Sep 1393 10:24
Last Modified:31 Jan 1400 13:24

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