جداسازی انانتیومرهای ترامادول به وسیله سیکلودکسترینهای پر سولفاته با کاپیلاری الکتروفورز

زندکریمی, مجید and شفاعتی, علیرضا and فروتن, سید محسن and حسنی علیائی, ناهید and اسلام پرست, آمنه (1384) جداسازی انانتیومرهای ترامادول به وسیله سیکلودکسترینهای پر سولفاته با کاپیلاری الکتروفورز. Daru ــ 13 (4). pp. 143-147. شاپا 1560-8115

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Separation of tramadol enantiomers by capillary electroforesis using highly sulfated cyclodextrines

English Abstract

In the pharmaceutical industry a continuing need for chiral resolution of drugs for various purposes and in diverse matrices exist. For these reasons, analysts may require a number of different separation systems capable of resolving a given pair of enantiomers. Highly sulfated cyclodextrins (HS-CDs) represent a relatively new class of chiral selectors in capillary electrophoresis (CE). In this investigation the use of HS-CDs as chiral selectors in CE for enantioseparation of tramadol, a highly potent analgesic, as the model drug and the influence of the type of selector and its concentration on enantiomeric resolution were studied. All of the available HSCDs ( α , β and γ ) could resolve tramadol enantiomers, but HS- γ -CD showed better resolution and a baseline resolution was achieved with this selector even at a concentration as low as 0.5% w/v. Additionally, ef fect of the buffer pH on the enantioresolution was studied. At low pH buffers, in which electroosmotic flow is low in CE, the negatively charged selector prevented the cationic tramadol to migrate out of the capillary even after a long analysis time of 60 minutes. However, at higher pH values (pH=7 or more), the electroosmotic flow is high enough to drag drug-selector complex toward the detector and a reasonable of the enantiomers of the drug was achieved.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مجید زندکریمی
نویسنده مسئول :علیرضا شفاعتی
نویسنده :سید محسن فروتن
نویسنده :ناهید حسنی علیائی
نویسنده :آمنه اسلام پرست
Additional Information:impact factor 1.11 Indexing: MEDLINE , PubMed , PubMed Central , Science Citation Index Expanded , Scopus , Index Copernicus , Biological Abstracts , CABI , CAS , Embase , Global Health Google Scholar
کلیدواژه ها (انگلیسی):tramadol enantiomers separation capillary electrophoresis
Subjects:QV pharmacology > QV 737 Pharmaceutical Biotechnology
Divisions:School of Pharmacy > Department of Pharmaceutical Biotechnology
ID Code:6035
Deposited By: Dr Ameneh Eslamparast
Deposited On:15 Aug 1393 08:59
Last Modified:15 Aug 1393 08:59

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