بررسی افزایش تعداد نسخ ژن زیر واحد کاتالیتیک آنزیم تلومراز در نمونه های پارافینه سرطان پستان در اردبیل

عنوان انگليسی

Investigation of the Increased Copy Number of the Telomerase Catalytic Subunit Genes in Breast Cancer Paraffin-Embedded Samples in Ardabil

خلاصه انگلیسی

Breast cancer is the most common cancer among the women and makes up one- third of cancers. Telomerase enzyme is a ribonucleoprotein with reverse transcriptase activity that adds TTAGGG repeated sequences to the ends of the chromosomes. The expression of the catalytic subunit of the enzyme (hTERT) in cancer cell lines and different human tumors is shown. So, in this study, the proliferation of copies of the hTERT gene in primary breast cancer was studied. Materials and Methods : Forty five Paraffin tissue samples from breast cancer patients and forty five Paraffin tissue samples from breast non-neoplastic patients was provided from Ardabil Imam Khomeini Hospital’s Pathology section and Plasma Laboratory.DNA samples were extracted with phenol-chloroform manually, then Real Time PCR was done with hTERT Forward, and Reverse primers and GAPDH Forward, and Reverse. The tumor sample was compared with the non- tumor one to investigate proliferation of copies of the hTERT gene as a prognostic indicator in patients with primary breast cancer. Results: Real time PCR curve analysis for hTERT and GAPDH genes in tumor samples compared with non-tumor samples showed that, there is no statistical deference’s between them (p=0.322). For data analysis Spss statistical software was used. Discussion: Our current studies on results of amplification hTERT gene, in comprising tumors samples with non-tumors is indication of early prognosis in primary breast cancer patients. While amplification of hTERT gene in breast cancer patients compared to the control group indicates that there is no statistical deference’s between them and it couldn’t be considered as prognosis factor for the primary breast cancer patients.

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