اسید رتینوئیک تمام ترانس بقا و آپوپتوز سلول های فیبروبلاست اطراف عصب محیطی را تغییر می دهد

نیاپور, نازیلا and نیاپور, علی and شیخکانلوی میلان, حمید and امانی, محمد and صالحی, حسین and نجف زاده, نوروز and غلامی, محمد رضا (1393) اسید رتینوئیک تمام ترانس بقا و آپوپتوز سلول های فیبروبلاست اطراف عصب محیطی را تغییر می دهد. Tissue and Cell ــ 47 (1). pp. 61-65. شاپا 0040-8166

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All trans retinoic acid modulates peripheral nerve fibroblasts viability and apoptosis

English Abstract

Objective: Following peripheral nerve injury, residing fibroblasts start to proliferate and accumulate atthe injury site and may participate in neuroma tissue evolution. Retinoic acid has been shown to regulatemany cellular processes and to display anti-proliferative and anti-fibrotic properties. The aim of thisstudy was to investigate the impact of all trans retinoic acid (ATRA) on rat peripheral nerve fibroblasts.Materials and methods: Peripheral nerve fibroblasts and C166 cells were treated with increasing dosesof ATRA (0.05 nM to 1 �M). The viability of cells was determined with 3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In addition, the number of peripheral nerve fibroblastswas counted after two days of ATRA treatment and alternatively up to the end of next week. Acridineorange/ethidium bromide double staining was implemented to morphologically visualize the possiblemechanism of cell death. For apoptosis, caspase 3/7 activity was measured using Caspase-Glo 3/7 assaykit.Results: MTT assay revealed that 0.05–1 nM of ATRA reduces fibroblasts viabilities. Then, almost a plateaustate was observed from 1 nM to 1 �M of ATRA exposure. Additionally, a deceleration in peripheralnerve fibroblasts growth was confirmed via cell counting. Quantification of acridine orange/ethidiumbromide staining displayed highly increased number of early apoptotic cells following ATRA adminis-tration. Amplified activation of caspase 3/7 was in favor of apoptosis in ATRA treated peripheral nervefibroblasts.Conclusion: The data from the present study demonstrate that ATRA could interfere in peripheral nervefibroblasts viabilities and induce apoptosis. Although more investigations are needed to be implemented,our in vitro results indicate that retinoic acid can probably help the regeneration of injured axon viareducing of fibroblasts growth.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :نازیلا نیاپور
نویسنده مسئول :علی نیاپور
نویسنده :حمید شیخکانلوی میلان
نویسنده :محمد امانی
نویسنده :نوروز نجف زاده
Additional Information:Impact Factor:(2014) 1.252 Abstracting and Indexing: Science Citation Index , MEDLINE, PubMed, EMBASE , Scopus , Cambridge Scientific Abstracts , Current Contents/Life Sciences Scisearch , Excerpta Medica , Current Awareness in Biological Sciences
کلیدواژه ها (انگلیسی):ATRA; Apoptosis; Peripheral nerve fibroblasts; Viability
Subjects:QS Human Anatomy
QY Clinical Pathology
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:6231
Deposited By: Dr Ali Niapour
Deposited On:04 Dec 1393 04:49
Last Modified:04 Aug 1394 11:14

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