اثر آپوپتوتیک رتینوئیک اسید تمام ترانس (ATRA) در سلول های سرطان معده از طریق کاهش بیان ژن Bcl2 و افزایش فعالیت کاسپاز3 صورت می گیرد

پات راد, الهام and نیاپور, علی and امانی, مجتبی (1393) اثر آپوپتوتیک رتینوئیک اسید تمام ترانس (ATRA) در سلول های سرطان معده از طریق کاهش بیان ژن Bcl2 و افزایش فعالیت کاسپاز3 صورت می گیرد. در: دوازدهمین کنگره بین المللی ایمونولوژی و آلرژی ایران, اردیبهشت 1393 9-12, تهران، ایران.

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Apoptotic effect of all-trans retinoic acid (ATRA) on gastric cancer cells through down-regulation of Bcl 2 and increased Caspase-3 activity

English Abstract

Background: Gastric cancer is one of the most common and lethal malignancies with high mortality rate in the world. Retinoic acid and its derivatives (Retinoids) have been utilized as potential chemopreventive and chemotherapeutic agents due to their anti-proliferative, anti-oxidant, pro-apoptotic and differentiation effects. Currently, all-trans retinoic acid (ATRA) is an approved drug for the treatment of acute promyeloid leukemia (APL) patients. One underlying mechanism by which ATRA carries out its anti-cancer effect is induction of apoptosis. Despite extensive studies, the main mechanism of apoptosis induction by ATRA has not been well elucidated. Caspases are central components of cell death machinery. Caspase-3, the executioner Caspase, is a key effector molecule of apoptosis and its activation is critical to induce programmed cell death in variety of cancer cells. Bcl2, a member of anti-apoptotic proteins, appears to protect cells from apoptosis by sequestering pro-apoptotic proteins or interfering with their activities. Method: In this study, AGS cells were treated by different concentrations of ATRA. Caspase-3 activation was measured by luminescent kit and was confirmed by RT-PCR. Moreover, down-regulation of Bcl2 expression was also evaluated by RT-PCR. Results: In this study we showed that ATRA treatment of gastric cell line (AGS) induced apoptosis through Caspase-3 activation. Furthermore, ATRA led to decrease in expression of Bcl2, an anti-apoptotic protein. Conclusion: ATRA is a potent agent to induce apoptosis in gastric cancer cells. These data suggests that induction of apoptosis through Caspase-3 activation is a novel mechanism of action for ATRA during therapeutic intervention in gastric cancer.

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده مسئول :الهام پات راد
نویسنده :علی نیاپور
نویسنده :مجتبی امانی
کلیدواژه ها (انگلیسی):Gastric cancer, Apoptosis, Caspase-3, Bcl2
Subjects:QU Biochemistry
QZ Pathology
WI Digestive System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:6248
Deposited By: Dr Ali Niapour
Deposited On:28 Oct 1393 07:22
Last Modified:28 Oct 1393 07:22

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