اثرات محافظتی ان-استیل سیستئین بر استرس اکسیداتیو القا شده با مالاتیون در سلولهای کبدی جداشده از موش صحرائی

مصطفی لو, سارا and عبدالهی, محمد and اقبال, محمد علی and سعیدی کوزه کنانی, نازلی (1390) اثرات محافظتی ان-استیل سیستئین بر استرس اکسیداتیو القا شده با مالاتیون در سلولهای کبدی جداشده از موش صحرائی. Advanced pharmaceutical bulletin ــ 2 (1). pp. 79-88. شاپا 2228-5881

Text - Published Version

Official URL: http://apb.tbzmed.ac.ir/AllIssues/Volume22012/2179...


Protective effect of NAC against malathion-induced oxidative stress in freshly isolated rat hepatocytes

English Abstract

PURPOSE: Induction of oxidative stress by Organophosphate compounds (OPs) has been previously reported. In the present work, the mechanism of protective effects of N-acetylcysteine as a glutathion (GSH) prodrug against malathion-induced cell toxicity was investigated. In this work, freshly isolated rat hepatocytes were used to determine the effect of NAC on malathion-induced cytotoxicity, formation of reactive oxygen species (ROS) and mitochondrial dysfunction. METHODS: Rat hepatocytes were isolated using collagenase perfusion and then cell viability, mitchondrial membrane potential (MMP) and ROS formation were determined using trypan blue exclusion, Rhodamine 123 fluorescence and fluorogenic probe, 2', 7' -dichlorofluorescin diacetate (DCFH-DA), respectively. RESULTS: Despite the protective effect of NAC on malathion-induced cell toxicity and MMP dysfunction, its efficacy against ROS formation was not adequate to completely protect the cells. CONCLUSION: Cytotoxic effects of malathion regardless of its cholinergic feature, is started with gradual free radical production but, the main factor that causes cell death, is mitochondrial dysfunction, so that reduction of ROS formation alone is not sufficient for cell survival, and the maintenance of mitochondrial integrity through different mechanisms is the most ameliorative factor specially at high levels of cell damage, as NAC seemed to protect cells with various fashions apart from ROS scavenging in concentrations higher than malathion's LC50.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سارا مصطفی لو
نویسنده مسئول :محمد علی اقبال
Additional Information:Indexing in: PubMed , Scopus , Index Copernicus , Ulrichs Web , embase ,Chemical Abstracts Google scholar , Ebsco
کلیدواژه ها (انگلیسی):Organophosphate Pesticides, Cytotoxicity, Oxidative Stress, Mitochondria, N-actylcycteine, Hepatocytes
Subjects:QV pharmacology > QV 600 Toxicology
QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:6266
Deposited By: Dr Sara Mostafalou
Deposited On:02 Nov 1393 05:14
Last Modified:02 Nov 1393 05:14

Repository Staff Only: item control page

Document Downloads

More statistics for this item...