title

القائ آپوپتوز و تعدیل فعالیت متالو پروتئیناز 2 در اثر واکنش الیگو داکسی نوکلئوتید CpG با TLR9

خرمی زاده, محمدرضا and حسین زاده, شهناز and صفوی فر, فرناز and سعادت, فرشید and عالی زاده, نسترن and فلک, رضا and جدلی, زهره and پزشکی, محمد (1385) القائ آپوپتوز و تعدیل فعالیت متالو پروتئیناز 2 در اثر واکنش الیگو داکسی نوکلئوتید CpG با TLR9. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY ــ 6 (3). pp. 107-114. شاپا 1502 -1735

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Title

Interaction of CpG-Oligodeoxynucleotides with Toll Like Receptor 9 Induces Apoptosis and Modulates Metaloproteinase-2 Activity in Human Intestinal Epithelium

English Abstract

Recent reports have indicated different effects of immunostimulatory sequences containing CpG-Oligodeoxynucleotides (ODN) on various immune cells. However, the exact role of CpG-ODN in human gut is unclear.In the present study ,we assessed potential effects of CpG –ODN on non lymphoid cell(intestinal epithelial cell line HT-29) on a dose-response and time –course basis. Intestinal epithelial cell line HT-29 was treated with CpG –ODN (CpG 2006) and lipopolysaccharide (LPS) at 5, 10, 25, 50 µg/ml and 1,5,10 µg/ml concentrations, respectively. Following treatments, dose-response and time-course cytotoxicity using a colorimetric method, Metaloproteinase-2(MMP-2)activity (using gelatin zymography)and apoptosis (using annexin-v flowcytometry method)assays were performed. Chloroquine treatment was also used for its inhibitory effect on endosomal acidification process to verify specific CpG –ODN and Toll Like Receptor 9 (TLR9) interactions.Cytotoxicity analysis of CpG –ODN showed that CpG –ODN increased significantly the proliferation of CpG –ODN treated cells ,as compared to untreated cells, at concentrations of 10-25 µg/ml (p<0.05).Overall MMP-2 activity analysis showed significant differences between treated and untreated cells. However, minimal changes were observed when MMP-2 activity was assessed per cell. Moreover, CpG –ODN treated cells demonstrated an increasing apoptosis rate of 0.8%, 6.46% and 14.21% at concentrations of 5,10,25 µg/ml, respectively. Collectively, our data indicated that intestinal epithelial cell line HT-29 is highly responsive to CpG effect in vitro and exhibits modified activities. The direct CpG –ODN and TLR9 interactions in HT-29 cells could provide new approaches in malignant tumor therapeutic strategies.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :محمدرضا خرمی زاده
نویسنده :شهناز حسین زاده
کلیدواژه ها (انگلیسی):Apoptosis , CpG-ODN , Cytotoxicity , HT29 , LPS MMP-2 , TLR9
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Immunology
ID Code:6288
Deposited By: Dr shahnaz hosseinzadeh
Deposited On:05 Nov 1393 10:05
Last Modified:04 Dec 1400 12:29

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