اثر ضد التهابی کلاولونیک اسید بر مدل التهاب القا شده با کاراژینین در موش صحرائی

Title

Clavulanic acid exhibits anti-inflammatory effects on carrageenan-induced paw edema model of inflammation in rats

English Abstract

The present study was designed to evaluate the anti-inflammatory effects of clavulanic acid, as a GCP-II inhibitor on carrageenan-induced paw edema in rats. Clavulanic acid was administered intraperitoneally (100, 200 or 300 mg/kg) 20 min before the subplantar injection of the carrageenan. Neutrophil infiltration (MPO activity), lipid peroxidation (MDA assay), IL1β, TNF-α and PGE2 levels were assessed in the paw tissue of clavulanic acid treated rats compared with the control rats. Results showed that intraperitoneal administration of clavulanic acid (200 or 300 mg/kg) significantly reduced maximum paw volume by 54.51% and 59.81% and total inflammatory response (AUC) by 53.17% and 59.13% in comparison with the control group, four hours after induction of inflammation (p<0.001). Intraperitoneal injection of clavulanic acid (100 or 200 mg/kg) resulted in a marked reduction of MPO activity in the inflamed paw by 79% and 82% respectively in comparison with the control group (p<0.01, p<0.001). Moreover, intraperitoneal clavulanic acid (200 mg/kg) reduced the MDA levels by 53.22% in comparison with the control group (p<0.001). The levels of IL-1β and PGE2 in the intraperitoneal clavulanic acid (300 mg/kg) treated rats, were reduced by 61% and 13% respectively in comparison with the control group (p<0.05). Overall we suggest that the anti-inflammatory properties of intraperitoneal clavulanic acid could be related to its inhibitory effect on GCP-II activity results in elevating NAAG level and reducing glutamate level increase.

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