بررسی اثرات الیگودزوکسی نوکلئوتیدها روی سلول های مدل سرطانی

خرمی زاده, محمدرضا and حسین زاده, شهناز and پزشکی, محمد and سعادت, فرشید (1385) بررسی اثرات الیگودزوکسی نوکلئوتیدها روی سلول های مدل سرطانی. در: هشتمین کنگره ایمونولوژی و آلرژی ایران, 26-28 اردیبهشت1385, علوم پزشکی تهران.

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Potential Effects of CpG-Oligodeoxynucleotides on Intestinal Epithelial Cells

English Abstract

Objectives: A pair of synthetic un-methylated CpG ODNs were used to assess the potential modulatory effects on non immune cell (epithelial cell line HT-29). Material & Methods: epithelial cell line HT-29 was treated with CpG ODN(CpG 2006) and lipopolysaccharide (LPS) at (5, 10, 25, 50 µg/ml) and (1,5,10 µg/ml) concentrations, respectively .Following treatments, dose-response and time-course cytotoxicity (using a colorimetric method), MMP-2 activity (using gelatin zymography)and apoptosis (using annexin-v flowcytometry method)assays were performed. to verify specific CpG effects , Chloroquine treatment was used for its inhibitory effect on endosomal acidification process. Results: Cytotoxicity analysis of CpG –ODN showed that CpG –ODN increases proliferation of cells at concentration 10-25 µg/ml. significant differences were observed between treated and untreated groups(p<0.05). MMP-2 activity analysis showed no significant differences between different groups of treated and untreated cells. Moreover, rate of apoptosis for CpG –ODN treated cells at 5,10,25 µg/ml concentrations was 0.8%, 6.46% and 14.21% , respectively, showing an increased apoptosis to necrosis ratio. Conclusion: intestinal epithelial cell line HT-29 is potentially highly responsive to CpG therapy upon TLR9 ligand stimulation in vitro.

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده اول :محمدرضا خرمی زاده
نویسنده مسئول :شهناز حسین زاده
کلیدواژه ها (انگلیسی):CpG-ODN , TLR9 , HT-29 , Apoptosis
Subjects:QU Biochemistry
QW Microbiology and Immunology
QZ Pathology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Immunology
ID Code:6291
Deposited By: Dr shahnaz hosseinzadeh
Deposited On:02 Nov 1393 03:49
Last Modified:04 Dec 1400 12:30

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