نقش CpG در مدل سلولی سرطان روده

خرمی زاده, محمدرضا and حسین زاده, شهناز and پزشکی, محمد and سعادت, فرشید and جدلی, زهره and صراف نژاد, عبدالفتاح and صفوی فر, فرناز and فلک, رضا and برهمه, آذر and میرشفیعی, عباس (1385) نقش CpG در مدل سلولی سرطان روده. در: دومین همایش بین المللی ژنتیک سرطان, 9 لغایت 11 آذز ماه سال 1385, دانشگاه علوم پزشکی تهران.

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The role of CpG in intestinal cancer cell model

English Abstract

Background: Recent reports have shown different effects of immunostimulatory sequences containing CpG motifs on various immune cells. However, the exact role of CpG-ODN in human gut is unclear .Aim: In the present study ,we assessed potential effects of CpG –ODN on non immune cell (epithelial cell line HT-29) on a dose-response and time –course basis. .Methods: epithelial cell line HT-29 was treated with CpG –ODN (CpG 2006) and lipopolysaccharide (LPS) at 5, 10, 25, 50 µg/ml and 1,5,10 µg/ml concentrations, respectively. Following treatments, dose-response and time-course cytotoxicity (using a colorimetric method), (MMP-2)activity (using gelatin zymography)and apoptosis (using annexin-v flowcytometry method)assays were performed. To verify specific CpG effects, Chloroquine treatment was used for its inhibitory effect on endosomal acidification process. Results: Cytotoxicity analysis of CpG –ODN showed that CpG –ODN increases proliferation of cells at concentrations of 10-25 µg/ml. significant differences were observed between treated and untreated groups(p<0.05(, MMP-2 activity analysis showed no significant differences between different groups of treated and untreated cells. moreover, rate of apoptosis for CpG –ODN treated cells at 5,10,25 µg/ml concentrations was 0.8%, 6.46% and 14.21% respectively , showing an increased apoptosis to necrosis ratio. Conclusion: intestinal epithelial cell line HT-29 is potentially highly responsive to CpG therapy in vitro and exhibits deviated cancer cell characteristic phenotypes.

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده اول :محمدرضا خرمی زاده
نویسنده مسئول :شهناز حسین زاده
کلیدواژه ها (انگلیسی):CpG , Cytotoxicity , HT-29 , Apoptosis
Subjects:QW Microbiology and Immunology
QZ Pathology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Immunology
ID Code:6296
Deposited By: Dr shahnaz hosseinzadeh
Deposited On:02 Nov 1393 03:53
Last Modified:04 Dec 1400 12:30

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