title

شناسائی آمیب های آزاد زی بیماریزا در آب های سطحی شهر شیراز، ایران

محمدی قلعه بین, بهنام and حاتم, غلامرضا and محمدپور, ایرج and قباخلو, نفیسه and فروغی پرور, فائزه (1393) شناسائی آمیب های آزاد زی بیماریزا در آب های سطحی شهر شیراز، ایران. Journal of health sciences and surveillance system ــ 2 (4). pp. 164-167. شاپا 2345-2218

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Title

Recognition of Pathogenic Free-Living Amoebae in the Surface Water in Shiraz, Iran

English Abstract

Background: There are many genera of free-living amoeba in the environment, but members of only four genera (Naegleria, Acanthamoeba, Balamuthia and Sappinia) have an association with human infection. Water, soil and air are main sources of infective types of these pathogenic organisms for human. Methods: Totally, 30 samples were collected from the surface water sources of Shiraz city, the capital of Fars province, during July and August 2009. The samples were filtered and their sediments were cultured on non-nutrient agar medium and seeded with non-pathogen Escherichia coli. Then, they were incubated at three different temperatures, 22˚C, 37˚C, and 44˚C. The media were checked with invert microscopy and amoebae were recognized by phase–contrast microscopy and observed by light microscopy after Trichrome staining. Polymerase chain reaction (PCR) was performed for molecular detection. Results: O f t h e 30 s a mpl e s , 2 9 we r e r e c og n i z e d mo r p h ol og i c a l l y a s Acanthamoeba, the characteristics of 20 of which were confirmed by PCR. The growth rate of amoeba in 22˚C was more than 37˚C. Eight of the samples grew at 44˚C, but flagellate forming test and PCR were negative for Naegleria fowleri. Two of them were identified morphologically as Balamuthia and Sappinia. Conclusion: Since Fars province is located in the subtropical region where there are a lot of parks and green areas with surface water, the potential risk of diseases caused by free-living amoebae should be considered. Further investigations about various aspects of these important opportunistic protozoa are recommended especially for establishment of appropriate prevention tools.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :بهنام محمدی قلعه بین
نویسنده مسئول :غلامرضا حاتم
نویسنده :ایرج محمدپور
نویسنده :نفیسه قباخلو
نویسنده :فائزه فروغی پرور
Additional Information:Indexed in: Index Copernicus , ISC , Google Scholar , Magiran , Iranmedex
Uncontrolled Keywords:آب های سطحی ، آکانتامبا ، شیراز
کلیدواژه ها (انگلیسی):Surface water , Acanthamoeba , Shiraz
Subjects:WA Public Health > WA 30 Social, Economic, and Environmental factor in public health
WA Public Health > WA 30 Social, Economic, and Environmental factor in public health

QX Parasitology
WA Public Health
WA Public Health
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:6450
Deposited By: Dr Behnam Mohammadi Ghalebin
Deposited On:16 Jan 1394 07:28
Last Modified:16 Jan 1394 07:28

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