اثر ترکیب درمانی با اریتروپویتین و ملاتونین روی آسیب ایسکمی و رپرفیوژن کلیه در رتهای نر

احمدی اصل, ناصر and بنایی, شکوفه and علی همتی, علیرضا and برادران, بهزاد and عظیمیان, احسان (1393) اثر ترکیب درمانی با اریتروپویتین و ملاتونین روی آسیب ایسکمی و رپرفیوژن کلیه در رتهای نر. Clinical and Experimental Nephrology ــ 18 (6). pp. 855-864. شاپا 1751-1342

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Effect of a combined treatment with erythropoietin and melatonin on renal ischemia reperfusion injury in male rats

English Abstract

Background Renal ischemia reperfusion (IR) is an important cause of renal dysfunction. It contributes to the development of acute renal failure. Oxidative damage from reactive oxygen species is considered to be the principal component involved in the pathophysiological tissue alterations observed during IR. The purpose of this study was to evaluate the effect of a combined treatment with erythropoietin (EPO) plus melatonin (MEL), which are known anti-inflammatory and antioxidant agents, in IRinduced renal injury in rats. Methods Wistar Albino rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h of reperfusion. MEL (10 mg/kg, i.p) and EPO (5000 U/kg, i.p) were administered prior to ischemia. After 24 h of reperfusion, blood samples were collected for the determination of superoxide dismutase (SOD), glutathione peroxidase (GPx), plasma levels of total antioxidant capacity (TAC), and malondialdehyde (MDA) and serum urea level. Also, renal samples were taken for histological evaluation. Results Ischemia reperfusion significantly increased urea, blood SOD, and GPx levels. Histological findings of the IR group indicated that there was increase in tubular and glomerular hyaline cast, thickening of Bowman capsule basement membrane, and renal impairment in the glomerular epithelium. Treatment with EPO and MEL significantly decreased blood SOD, GPx, and urea levels and increased TAC level. In the EPO ? MEL group, while the histopathological changes were lower than those in EPO group, they were the same as MEL group. Conclusion EPO and MEL combination treatment exerted more nephroprotective effects than EPO treatment and nearly had protective effects similar to MEL treatment.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :ناصر احمدی اصل
نویسنده مسئول :شکوفه بنایی
نویسنده :علیرضا علی همتی
نویسنده :بهزاد برادران
نویسنده :احسان عظیمیان
Additional Information:Impact Factor (2014) 2.02 Indexed in: ISI, Pubmed/Medline,Index Medicus, Scopus, EMBASE, Google Scholar, EBSCO
کلیدواژه ها (انگلیسی):Ischemia reperfusion injury, Antioxidants, Melatonin, Erythropoietin, Kidney
Subjects:QT physiology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:6483
Deposited By: Dr shokofeh banaei kivi
Deposited On:23 Jan 1395 07:32
Last Modified:22 May 1399 07:31

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