سیستمی کارآمد جهت جداسازی و کشت سلول های شوان عصب محیطی موش صحرایی بالغ

نیاپور, نازیلا and محمدی قلعه بین, بهنام and گل محمدی, محمد قاسم and غلامی, محمد رضا and امانی, محمد and نیاپور, علی (1393) سیستمی کارآمد جهت جداسازی و کشت سلول های شوان عصب محیطی موش صحرایی بالغ. Cytotechnology ــ online . pp. 1-8. شاپا 9069-0920

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Official URL: http://link.springer.com/article/10.1007/s10616-01...


An efficient system for selection and culture of Schwann cells from adult rat peripheral nerves

English Abstract

Schwann cells (SCs), the supporting cells of the peripheral nerves, are indispensable for regenerating the peripheral and central nervous system. Copious preparation of these cells in a well-defined manner is to be a privileged position. SCs cultivation is overwhelmed by contaminating fibroblasts which are often outgrowing as the predominant cell type in an in vitro culture. This study introduces a technically simple and efficient procedure for SCs isolation and enrichment based on implementing recombinant and defined supplements. Collected adult rat sciatic nerves were cultured for 10 days as in vitro predegeneration. After dissociation and plating, the medium changed to knockout serum replacement supplemented DMDM/F12 medium containing various growth factors. The whole procedure took 3 weeks and SCs purity was then evaluated through implementing specific cytoplasmic and membranous markers. The viability of enriched SCs were evaluated by MTT assay. Within 10 days, over 99 % homogenous SCs were achieved and confirmed through immunofluorescence staining and flow-cytometry for P75NTR and S100 markers, respectively. MTT data revealed that the viability and metabolic activities of purified SCs were increased in expansion medium. This study provides a technically easy and efficient method with the benefits of not utilizing bovine serum or other animal products for SCs isolation and enrichment.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :نازیلا نیاپور
نویسنده :بهنام محمدی قلعه بین
نویسنده :محمد قاسم گل محمدی
نویسنده :محمد رضا غلامی
نویسنده :محمد امانی
نویسنده مسئول :علی نیاپور
Additional Information:Impact Factor: (2014) 1.752 Indexed in: ISI, Scopus, EMBASE
کلیدواژه ها (انگلیسی):Schwann cell, Rat sciatic nerve, KOSR, Defined culture
Subjects:QS Human Anatomy
QV pharmacology
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:6629
Deposited By: Dr Ali Niapour
Deposited On:21 Aug 1394 05:33
Last Modified:31 Jan 1400 13:02

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