آلیسین از سیر تشکیل بیوفلیم و فعالیت آنزیم اوره آز پروتئوس میرابلیس را در شرایط آزمایشگاهی مهار می کند

عنوان انگليسی

Allicin from garlic inhibits the biofilm formation and urease activity of Proteus mirabilis in vitro

خلاصه انگلیسی

Several virulence factors contribute to the pathogenesis of Proteus mirabilis. This study determined the inhibitory effects of allicin on urease, hemolysin and biofilm of P. mirabilis ATCC 12453 and its antimicrobial activity against 20 clinical isolates of P. mirabilis. Allicin did not inhibit hemolysin, whereas it did inhibit relative urease activity in both pre-lysed (half-maximum inhibitory concentration, IC50=4.15μg) and intact cells (IC50=21μg) in a concentration-dependent manner. Allicin at sub-minimum inhibitory concentrations (2–32μg/mL) showed no significant effects on the growth ofthe bacteria (P>0.05), but it reduced biofilm development in a concentration-dependent manner (P<0.001). A higher concentration of allicin was needed to inhibit the established biofilms. Using the microdilution technique, the MIC90 and MBC90 values of allicin against P. mirabilis isolates were determined to be 128 and 512μg/mL respectively. The results suggest that allicin could have clinical applications in controlling P. mirabilis infections

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