پیش شرطی کردن با استرس اکسیداتیو، بقای سلولهای بنیادی مزانشیمی مشتق از مغز استخوان را افزایش می دهد.

خوش لحنی, نسرین and میرزاپور, طوبی and محمدزاده وردین, محمد and سقا, محسن (1394) پیش شرطی کردن با استرس اکسیداتیو، بقای سلولهای بنیادی مزانشیمی مشتق از مغز استخوان را افزایش می دهد. در: International Congress on Stem Cells and Regenerative Medicine, 20-22 May 2015, Mashhad, Iran.

Text - Published Version

Official URL: http://www.mashhadstemcell.com/


Preconditioning with Oxidative Stress Increases the Survival Rate in Bone Marrow-derived Mesenchymal Stem Cells

English Abstract

Introduction: Mesenchymal stem cells are multipotent stem cells having capacity for differentiation to various types of cells such as osteocyte, adipocyte, and chondrocytes. They can be easily isolated from several organs without any prominent invasive procedure. Both differentiation ability and immunomodulatory effects of the bone marrow–derived mesenchymal stem cells (BM-MSCs) make them to be a promising source for tissue repairing and vehicles of cell-based gene therapy. Moreover, the homing properties of BM-MSCs offer them to be a prospective tool for augmenting of the tissue regeneration. Nevertheless, some disadvantages including low survival rate following transplantation will be resulted by a lot of oxidative stress caused by hypoxia/inflammation in damaged tissue. These conditions restrict the broad applications of BM-MSCs for clinical purposes. Therefore, each strategy protecting BM-MSCs from premature death and improving the survival rate will be valuable for their therapeutic aims. Preconditioning denotes the cell exposure to minute rate of oxidative stress for a short period of time, reinforcing the cells against the higher level of stress. Literature review: Preconditioning with oxidative stress induced by H2O2 is one of the successful strategies to increase the cell viability. Previous studies have demonstrated preconditioning of PC12 cells with H2O2 increased their survival rate through reducing the intracellular reaction oxygen species (ROS) and rising the mitochondrial membrane potential (MMP). Moreover, H2O2 preconditioned BM-MSCs significantly showed higher survival percentage against the harmful conditions and prevention of apoptosis. Recent studies have also reported that preconditioned–based cell therapy in mice with infracted myocardium enhances the neovascularization in heart and improves its function through overexpression of HGF and IL-6 as well as VEGF upregulation followed by high expression of HIF-1α protein. Conclusion: H2O2 causes oxidative stress in cells by producing many free radicals such as superoxide (O2-) and hydroxyl radical (OH·) that may lead to apoptosis. However, low concentrations of H2O2 for a short period of time protect BM-MSCs against oxidative stress and increase their survival rate both in vivo and in vitro.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :نسرین خوش لحنی
نویسنده :طوبی میرزاپور
نویسنده :محمد محمدزاده وردین
نویسنده مسئول :محسن سقا
Additional Information:شماره پوستر: P274
کلیدواژه ها (انگلیسی):Mesenchymal Stem Cells, Survival, Oxidative Stress, H2O2
Subjects:QT physiology
QU Biochemistry
WH Hemic and Lymphatic System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:6741
Deposited By: Dr Mohammad Mohammadzadeh
Deposited On:28 Mar 1394 02:50
Last Modified:28 Mar 1394 02:50

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