title

مدولاسیون ژن (TNF-α) در آسیب ریوی ناشی از اشعه با استفاده از اسید گلیسریزیک

رفاهی, سهیلا and پورعیسی, مسعود and زیرک, محمدرضا and حدادی, غلامحسين (1394) مدولاسیون ژن (TNF-α) در آسیب ریوی ناشی از اشعه با استفاده از اسید گلیسریزیک. journal of medical physics ــ 40 (2). pp. 95-101. شاپا 6203-0971

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Title

Modulation expression of tumor necrosis factor α (TNF-α) in the radiation-induced lung injury by glycyrrhizic acid

English Abstract

To evaluate the ability of glycyrrhizic acid (GLA) to reduce the tumor necrosis factor α (TNF-α), release on messenger ribonucleic acid (mRNA) and protein production in the lungs using GLA in response to irradiation were studied. The animals were divided into four groups: No treatment (NT group), GLA treatment only (GLA group), irradiation only (XRT group), and GLA treatment plus irradiation (GLA/XRT group). Rats were killed at different time points. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used to evaluate the mRNA expression of TNF-α in the lungs (compared with non-irradiated lungs). An enzyme-linked immunosorbant assay (ELISA) assay was used to measure the TNF-α protein level. The TNF-α mRNA expression in the lungs of the XRT rats was clearly higher at all-time points compared to the NT rats. The TNF-α mRNA expression in the lungs of the GLA/XRT rats was lower at all-time points compared to the XRT rats. Release of the TNF-α on protein level in the lungs of the XRT rats increased at all-time points compared to the NT rats. In contrast to the XRT rats, the lungs of the GLA/XRT rats revealed a reduction on TNF-α protein level at 6 h after irradiation. This study has clearly showed the immediate down-regulation of the TNF-α mRNA and protein production in the lungs using GLA in response to irradiation.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سهیلا رفاهی
نویسنده :مسعود پورعیسی
نویسنده :محمدرضا زیرک
نویسنده مسئول :غلامحسين حدادی
Additional Information:Indexing in: Pubmed, SCOPUS, Google Scholar, Index Copernicus, Indian Science Abstracts, Excerpta Medica / EMBASE
کلیدواژه ها (انگلیسی):Glycyrrhizic acid ,radiation induced lung damage , tumor necrosis factor α
Subjects:QY Clinical Pathology
WF Respiratory System
WN Radiology . Diagnostic Imaging
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Medical Physics
ID Code:6745
Deposited By: MS Soheila Refahi
Deposited On:05 May 1394 06:00
Last Modified:08 Jun 1395 04:26

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