تاثیر هم کشتی سلول های بنیادی مزانشیم مشتق از مغزاستخوان با سلول های k562 در القاء مقاومت در برابر تیمار کردن با سیتارابین در سلول k562

Title

Co-culture of human bone marrow derive dmesenchymal stem cells with k562 cells conferred resistance to k562 cells against cytarabine treatment

English Abstract

Background: Human mesenchymal stem cells (H-MSCs) are population of non-hematopoietic cells that have many applications in medicine most notably in cell therapy. Despite the many advantages, there are still concerns regarding the use of these cells such as induction of drug resistance. Objective: This study was performed to evaluate the effects of MSCs on k562 cells (human erythroleukemia cell line) in terms of cytotoxicyty or apoptosis following treatment with a well-known chemotherapy agent i.e. cytarabine Materials & Methods: H-MSCs were isolated from health volunteers and characterized by flowcytometery. MSCs were co-cultured with k562 cell line in presence of serial concentrations of cytarabine. As a control, K562 cells were also treated with cytarabine without cultivation with H-MSCs. The cell viability was determined by trypan blue exclusion test and MTT assay. Apoptosis was studied by ELISA kit. Results: Cultivation of MSCs with k562 in the presence of cytarabine conferred resistance to k562.In other words, higher concentration of cytarabine was required to induce cytotoxicity when k562 cells cultivated with MSCs. Cytarabine also induces apoptosis cells in k562 in the concentration of (0.1-0.4μg) however and interestingly apoptosis cells were lower when they co-cultured with MSCs. Conclusions: This study indicated that MSCs confer resistance to erythroleukemia cell line following treatment with chemotherapy agent and prevent leukemic cells apoptosis. Our finding may open new window why erythroleukemia cell become resistance to chemotherapy and may suggest new strategy for treatment of them.

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