تاثیر نگهداری کوتاه مدت روی تحرک، شاخصهای اکسیداتیو و محتوای آدنوزین تری فسفات اسپرم تاس ماهی ایرانی

Title

Effects of short-term storage on the motility,oxidative stress, and ATP content of Persian sturgeon(Acipenser persicus) sperm

English Abstract

The effective short-term storage of semen is essential when processing multiple spermsamples and when semen must be transported from collection sites to hatcheries forthe fertilization of ova, or to laboratories for cryopreservation. In the present study, thespermatozoa of Persian sturgeon (Acipenser persicus) were used to evaluate the effects ofshort-term storage on quality parameters (the percentage of motile cells and the total periodof sperm motility), oxidative stress indices, and the ATP content. Spermatozoa cells exhib-ited >50% motility during 6 days of storage where the average total duration of spermmotility varied from 376.42 ± 80.86 s initially to 19.28 ± 10.96 s after 6 days. No motilespermatozoa were recorded after 9 days of storage. The levels of oxidative stress indices(TBARS and CP) and antioxidant activity (SOD) increased significantly with the storage time.The ATP content also decreased significantly after 2 days of storage. The results of this studymay facilitate successful reproduction management and cryopreservation protocols for thisendangered fish.

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