پیش تجویز زردچوبه از استرس اکسیداتیو و آسیب کبدی القاء شده توسط متوترکسات محافظت می کند

رضایی مقدم, عادل and توتونچی, سهیل and ناموران عباس اباد, علی and یزدی, مینا and بنیادی, فاطمه and مهاجری, داریوش and مأذنی, محمد and مرزبان, حسن and مارک, جی لوس and قوامی, سعید (1394) پیش تجویز زردچوبه از استرس اکسیداتیو و آسیب کبدی القاء شده توسط متوترکسات محافظت می کند. BMC Complementary and Alternative Medicine ــ 15 (246). pp. 1-13. شاپا 6882-1472

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Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress

English Abstract

Background: Methotrexate (MTX) is an antimetabolite broadly used in treatment of cancer and autoimmune diseases. MTX-induced hepatotoxicity limits its application. We investigated hepatoprotective effects of turmeric in MTX-induced liver toxicity. Methods: All experiments were performed on male Wistar albino rats that were randomly divided into six groups. Group one received saline orally for 30 days (control group), groups two and three received turmeric extract (100, 200 mg/kg respectively) orally for 30 days, group four received single dose, of MTX IP at day 30, groups five and six received turmeric extract 100 and 200 mg/kg orally respectively for 30 days and single dose of methoterxate IP (20 mg/kg) at day 30. Four days after MTX injection animals were sacrificed and evaluated. Blood ALT and AST (indicators of hepatocyte injury), ALP and bilirubin (markers of biliary function), albumin (reflect liver synthetic function) as well as the plasma TAS concentration (antioxidant defenses) were determined. The cellular antioxidant defense activities were examined in liver tissue samples using SOD, CAT, and GSH-Px for the oxidative stress, and MDA for lipid peroxidation. In addition, liver damage was evaluated histopathologically. Results: MTX significantly induced liver damage (P < 0.05) and decreased its antioxidant capacity, while turmeric was hepatoprotective. Liver tissue microscopic evaluation showed that MTX treatment induced severe centrilobular and periportal degeneration, hyperemia of portal vein, increased artery inflammatory cells infiltration and necrosis, while all of histopathological changes were attenuated by turmeric (200 mg/kg). Conclusion: Turmeric extract can successfully attenuate MTX-hepatotoxicity. The effect is partly mediated through extract’s antinflammatory activity.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :عادل رضایی مقدم
نویسنده :سهیل توتونچی
نویسنده :علی ناموران عباس اباد
نویسنده :مینا یزدی
نویسنده :فاطمه بنیادی
نویسنده :داریوش مهاجری
نویسنده :محمد مأذنی
نویسنده :حسن مرزبان
نویسنده :جی لوس مارک
نویسنده مسئول :سعید قوامی
Additional Information:Impact Factor (2014): 2.02 Indexed in: ISI, Pubmed/Medline, Index Medicus, Scopus,
کلیدواژه ها (انگلیسی):Lipid peroxides, Total antioxidant status, Antioxidant enzymes, Serum liver biomarkers, Hepatocellular injury, Cell death
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
ID Code:6899
Deposited By: Dr Mohammad Mazani
Deposited On:24 Jun 1394 05:33
Last Modified:20 May 1399 08:51

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