سنجش مبودیپین با کروماتوگرافی مایع با کارکرد عالی: کاربری در مطالعات فارماکوکینتیک

بهلولی, شهاب and کیهانفر, فریبرز and محمودیان, مسعود (1380) سنجش مبودیپین با کروماتوگرافی مایع با کارکرد عالی: کاربری در مطالعات فارماکوکینتیک. Journal of Pharmacy and Pharmaceutical Sciences ــ 4 (3). pp. 244-247. شاپا 1482-1826

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High performance liquid chromatography of mebudipine: Application to pharmacokinetic study

English Abstract

Purpose: To develop a high performance liquid chromatography system for the determination of a new 1,4-dihydropyridine, mebudipine, in rabbit plasma. Methods: To 1 ml of rabbit plasma was added internal standard (dibudipine) and 0.5 ml of 1 M NaOH. Mebudipine and internal standard were extracted to 5 ml ethyl acetate, evaporated under slow stream of nitrogen. The residue was reconstituted in 200 μ1 mobile phase and 20 μ1 of aliquots were injected into a HPLC system equipped with 4.6x250 mm i.d. C18 analytical column. Mobile phase consisted of methanol (70%), water (25%) and acetonitril (5%) and its flow rate was 1 ml/min. Results: There were no interfering peaks from endogenous components in blank plasma chromatograms. Standard curves were linear (r2>0.99) over 10 to 500 ng/ml. The extraction efficiency was >90% and the minimum quantifiable concentration was 10 ng/ml (CV<10%). Conclusion: A suitable, convenient and simple HPLC assay for pharmacokinetic study of mebudipine in rabbits was developed.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :شهاب بهلولی
نویسنده مسئول :مسعود محمودیان
Additional Information:تعداد ارجاعات: 5 ، Impact Factor: 0.8 در سال 2001
Uncontrolled Keywords:فارماکوکینتیک ، مبودیپین ، کروماتوگرافی مایع با کارکرد عالی
کلیدواژه ها (انگلیسی):pharmacokinetics , mebudipine , high performace liquid chromatography
Subjects:QV pharmacology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Pharmacology
ID Code:693
Deposited By: Dr Shahab Bohlooli
Deposited On:25 Oct 1388 16:04
Last Modified:25 Nov 1391 08:49

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