title

کارایی رقیق کننده حاوی گلوکز- متانول برای انجماد اسپرم فیل ماهی

آراملی, محمد صادق and گلشاهی, کریم and نظری, رجب محمد and آراملی, سلیم and بنان, اشکان (1394) کارایی رقیق کننده حاوی گلوکز- متانول برای انجماد اسپرم فیل ماهی. Animal Reproduction Science ــ 162 . pp. 37-42. شاپا 4320-0378

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Title

Effectiveness of glucose–methanol extender forcryopreservation of Huso huso spermatozoa

English Abstract

The present approach was designed to evaluate the methanol–glucose extender effects onsperm cryopreservation in beluga sturgeon, Huso huso. Sperm quality was examined bymeasuring post-thaw sperm motility and fertilizing rate at hatching stage. We first testedthe effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30 M) in a methanol extender onpost-thaw sperm motility. The optimal cryopreservation conditions were found to be 0.2 Mglucose in the extender. Then, motility and fertilization rates of sperm cryopreserved with0.2 M glucose and 10% methanol (GM) were compared to Tris–sucrose–KCl in 10% methanolextender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 15 and30 min equilibration in GM extender before and after cryopreservation were measured.Higher post-thaw sperm motility duration and percentage as well as fertilization rate wereobtained with the GM extender when compared to TSKM extender. Equilibration of sperm inextender did not affect the motility quality of either fresh-diluted or frozen/thawed sperm,while fertilization rate showed a significant decline alone after 30 min of post-thaw storage.Our results indicated that the use of a simple extender consisting of 0.2 M glucose in 10%methanol can be an alternative cryopreservation method to those previously described forsturgeons.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :محمد صادق آراملی
نویسنده :کریم گلشاهی
نویسنده :رجب محمد نظری
نویسنده :سلیم آراملی
نویسنده :اشکان بنان
Additional Information:Impact Factor(2014)1.511 Indexed in: ISI, Pubmed/Medline/Index Medicus, Scopus, Animal Breeding Abstracts, Bibliography of Reproduction, Current Contents/Agriculture, Biology & Environmental Sciences, Biological Abstracts, Current Awareness in Biological Sciences Original Research Article
کلیدواژه ها (انگلیسی):Huso huso, Cryopreservation, Sperm motility, Fertilization, Glucose, Extender
Subjects:QT physiology
WP Gynecology
Divisions:Alavi Educational and Treatment Center > Medicine Laboratory-Alavi Educational and Treatment Center
ID Code:6978
Deposited By: Mr salim Aramli
Deposited On:27 Nov 1394 06:58
Last Modified:26 Jan 1397 10:42

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